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Properties of aminoclay complexes and biologically active substances
Dušek, Jakub ; Pekař, Miloslav (referee) ; Mravec, Filip (advisor)
This paper builds on previous research of aminoclay complexes in undergraduate studies. Theoretical part deals with study of current problems of aminoclay complexes with bioactive substances and the choice of substances for complexing with aminoclay. The experimental part consists of preparation of aminoclay complexes with selected bioactive agents at various concentrations. Verification of binding of bioactive agents to the aminoclay matrix was performed by Elemental Analysis (EA) and Fourier-transform infrared spectroscopy (FT-IR). For finding of the bound amount of bioactive substance were used the Ultra Performance Liquid Chromatography (UPLC®) and analysis of the cytotoxic properties of the formed complexes by used by the MTT assay. The main motivation of this study is to create new complexes with improved characteristics that would replace existing forms of substances used in pharmaceutical and biomedical applications.
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Draft solution of unified industrial communication system (ICS) for the foundry object
Dušek, Jakub ; Jordán, Vilém (referee) ; Sedlák, Petr (advisor)
This bachelor thesis deals with the design of a complex network infrastructure for the Brno foundry, which is supposed to replace the inadequate infrastructure within the foundry. In the second part of the thesis are explained the concepts of computer network, network topology, Ethernet, section of cabling system and basic concepts needed to understand network infrastructure design. The third part deals with the analysis of the current situation and sets out recommendations for a new network infrastructure. The last part deals with the complex design of the network infrastructure in the foundry premises.
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Design and implementation of control applications capable to connect laboratory MES system
Dušek, Jakub ; Pásek, Jan (referee) ; Kaczmarczyk, Václav (advisor)
The goal of this bachelor thesis is to understand the ISA-95 standard and the manufacturing executing system (MES) based on this standard. Control application of discrete manufacturing controlling for PLC is then created and applied on a school device model. The thesis explains the ISA-95 standard and its influence on MES. The division and functions of MES according to ISA-95 are further described and explained. The theoretical part, the application of discrete manufacturing controlling for PLC is implemented on a drill model, simulating a segment of drilling. Structure of the software has been implemented in a way to correspond to the requirements given by MES and to be easily built upon.
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Complexes of aminoclay and selected organic compounds
Dušek, Jakub ; Pekař, Miloslav (referee) ; Mravec, Filip (advisor)
This paper describes the preparation of aminoclay and complexes of aminoclay with selected bioactive substances. The theoretical part deals with the study of aminoclay and substances suitable for complexes and suitable modification of preparation these complexes. Experimental part of this paper consists of preparation of aminoclay complexes and verification of their formation. In order to verify the formation of the complex by binding of bioactive compounds to the free amino groups of aminoclay were used methods FT-IR (Fourier transform infrared spectroscopy) and XRD (X-ray diffraction). For selected substances, binding to the aminoclay would improve their properties, which was the main motivation for the research. The resulting complexes could be more effective by replacing existing substances used in the pharmaceutical industry and biomedical applications.
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Administrative and economy of the so called Aztec Empire
Dušek, Jakub ; Křížová, Markéta (advisor) ; Binková, Simona (referee)
(in English): This thesis examines and tests the hypothesis whether political entity, in literature traditionally referred to as the "Aztec Empire", was actually an empire equivalent to European states of that age. Based on this hypothesis there are two objectives in this paper. The first one is to answer the question of what principles really stood on this political entity, how the relationships between the core of the empire and other provinces varied and what affected these relationships. The second objective is to examine the economic system of the Aztec empire because that was extensively intertwined with its political actions and influenced them. All three components of the Aztec economy are analyzed, thus supplying the Triple Alliance on its own (intensive agriculture, trade) and extracting tributes from conquered territories. Attention is also paid to the history of "Aztec empire" and its Mesoamerican context. The main sources are edited sources from early colonial times, depicting the situation before the arrival of Europeans and secondary literature, stemming mainly from ethnohistorical research.
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Puerto-Rican Spanish with a Focus on Lexis
Dušek, Jakub ; Mištinová, Anna (advisor) ; Zavadil, Bohumil (referee)
(in English): This Bachelor's Thesis examines the Puerto Rican Spanish vocabulary, which contains a large number of typical features, generally applicable to American Spanish (on a lexical level). A brief overview of the history of the country is also included, as each historical period has had some impact on its language. The paper then describes the theoretical background of every typical feature of the Puerto Rican lexicon, especially its origin or definition. This is followed by a practically focused part, in which specific examples for each aspect in vocabulary. From this chapter conclusions are then drawn summarizing the most important findings of the examined lexical features. The last described area is the situation of English on the island and the presence of Puerto Rican Spanish in the United States. I focused on the specific case of the interaction of both official languages, thus involving the use of anglicisms and Spanglish speech. In conclusion, I have tried to show, inter alia, the basis for the future development of this issue in Puerto Rico.
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Rating the resistance of tomato (\kur{Lycopesicum annum }L.) cultivars to late blight (\kur{Phytophthora infestans})
DUŠEK, Jakub
The aim of this thesis was to evaluate resistent varieties of tomato in the term of susceptibility to late blight. Late blight , caused by P. infestans, is the most destructive foliar disease of tomato. Some new sorts declare the resistence to late blight. The aim of our thesis was to evaluate how these varieties perform in Czech republic. It was established experiment with 14 resistent and 2 control varieties. The leaves of the plants were examined routinely for symptoms of late blight and the severity of them was recorded. The varieties Mountain Magic, De Barao, Cherry Bomb, Tomato Des Comodores, Matts Wild Cherry a Plum Regal proved high level of resistence to late blight. The varieties Phantasia, Phytovia and Mountain Merit showed lower level of resistence. Growing new varieties, with resistence to late blight can enable the reduction of fungicidal protection of tomatoes and for organical and hobby growers to make possible growing tomatoes without use of pesticides.
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Introducing targeted epigenetic changes in Solanum tuberosum and Nicotiana benthamiana by plant viruses
Dušek, Jakub ; Ryšánek, Pavel (advisor) ; Barbora, Barbora (referee)
In this thesis we have focused on the induction of targeted epigenetic changes using modified Potato Virus X vector (PVX, Potato virus X). The gene coding sequence phytoene desaturase (PDS) was isloated from Solanum tuberosum and Nicotiana benthamiana plants using PCR. Primers were specifically designed as universal for both plant species and their Tm temperatures was in the range of 5 °C. The primers also added flanking restriction sites XhoI and ClaI to simplify cloning of PDS PCR products into full length PVX viral vector in AS orientation. The PVX viral vector pGR106 was later introduced into the plants using Agrobacterium tumefaciens. Infection led to the degradation of the target transcript and to efficient gene silencing (PTGS posttranscriptional gene silencing). The process has been manifested by complete or partial fading of a green plant tissue phenotype..
Some of the plants were aslso inoculated in in vitro conditions, for which a novel technique has been implemented.
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Implementation of VBA for processes automation in university environment
Dušek, Jakub ; Benáčanová, Helena (advisor) ; Fortinová, Jana (referee)
This bachelor thesis brings a description of application development that optimizes and automates certain operations at IT Help desk at University of New York in Prague. MS Excel and programming language Visual Basic for Application (VBA) are used for development of aforementioned application The first part of the thesis is focused on environment description and inner factors. Thanks to the extensive description of relevant processes it was possible to identify its bottlenecks and optimize them with the developed application. The second part is dedicated to solution of this application. Here I describe its usage by means of screenshots. I also introduce important parts of the source code which is described subsequently.
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