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Study of etiopathology of mitochondrial disorders
Rákosníková, Tereza ; Tesařová, Markéta (advisor) ; Pecina, Petr (referee) ; Kalous, Martin (referee)
Mitochondrial disorders are a clinically, biochemically and genetically heterogeneous group of inherited disorders with a prevalence of about 1:5 000 live births. A common sign of those disorders is disruption of mitochondrial energetic metabolism. To this day, more than 400 genes have been associated with mitochondrial disorders, but 45% of patients are still without a genetic diagnosis. Using next-generation sequencing, new candidate genes or variants are found. To confirm the causality of those newly found genes or variants, biochemical characterisation using a plethora of various methods is necessary. The first aim of this thesis was to study the function of ACBD3 protein on mitochondrial energetic metabolism in non-steroidogenic cells HEK293 and HeLa and to confirm the causality of the ACBD3 gene in a patient with combined oxidative phosphorylation (OXPHOS) deficit. The second aim was to confirm the causality of two novel variants in MT-ND1 and MT-ND5 genes, which encode structural subunits of complex I (CI) of the respiratory chain. The third aim of the thesis was to study the formation of supercomplexes (SCs) in patients with rare metabolic diseases. Using functional studies, we showed in this thesis that ACBD3 protein has no essential function in mitochondria but plays an important role in...
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Zpracování podnikatelského záměru vybraného produktu
TESAŘOVÁ, Marie
This bachelor's thesis deals with the creation of a business plan for the establishment of a cake factory called "Dortíkárna". The theoretical part of this thesis describes the basic terms of business, the initial steps in establishing a secondary trade, marketing mix, sociological research, and the definition of confectionery. The thesis then deals with the realization of the business plan based on analyses and applied to the situation. Design of the promotional website and social network advertising is also covered. Finally, there is an evaluation of the entire business plan.
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Quantitative 3D characterization of biological structures by X-ray computed microtomography
Tesařová, Markéta ; Vaňhara,, Petr (referee) ; Oberta,, Peter (referee) ; Zikmund, Tomáš (advisor)
Moderní vývojová biologie využívá široké spektrum metod k analyzování studovaných struktur. Multioborové týmy v poslední době prosazují využívání 3D přístupu k porozumění biologickým procesům. Jedna z hlavních překážek při využívání 3D zobrazovacích technik v biologii je nedostatek metodologie pro kvantifikování pozorovaných jevů, které často bývají příčinou vývojových či genetických onemocnění. V posledních letech se v biologických výzkumech rozšířilo využití rentgenové počítačové mikrotomografie (mikroCT). Technika mikroCT dokáže poskytnout izotropní rozlišení až 1 µm a při správné přípravě vzorku širokou škálu tkáňové diferenciace. Biologické struktury jsou však rozmanitých velikostí a tvarů a každá struktura vyžaduje specifický přístup v oblasti zpracování dat. Tato práce se zabývá využitím mikroCT pro zobrazování, ale především 3D charakterizaci biologických struktur ex-vivo. Zvláštní důraz je kladen na analýzu měkkých tkání v hlavách myších embryí a zobrazování různých druhů mloků se zaměřením na jejich regenerační schopnosti. Tato práce popisuje relevantní informace pro uživatele mikroCT zabývajícími se biologickými projekty: To zahrnuje přípravu vzorku pro měření, výběr vhodných parametrů k samotnému měření, a především analýzu vzniklého 3D datasetu. Nejnovější technologické pokroky ve 3D zobrazovaní byly studovány a testovány na konkrétních projektech vývojové biologie, kde 3D informace získaná pomocí mikroCT hrála klíčovou roli.
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Specific training and education of gifted children
Tesařová, Monika ; Dvořáčková, Hana (advisor) ; Nováková, Ivana (referee)
UNIVERZITA KARLOVA V PRAZE Husitská teologická fakulta BAKALÁŘSKÁ PRÁCE Specifika vzdělání a výchovy nadaného dítěte Vedoucí práce: Autor: PhDr. Hana Dvořáčková Monika Tesařová Praha 2012 CHARLES UNIVERSITY IN PRAGUE Hussite Theological Faculty BACHELOR THEIS Specific training and education of gifted children The work supervisor: Autor: PhDr. Hana Dvořáčková Monika Tesarova Prague 2012 Děkuji PhDr. Haně Dvořáčkové za její podněty a rady, které mi jako vedoucí mé bakalářské práce poskytovala při jejím zpracovávání. Prohlašuji, že jsem tuto bakalářskou práci s názvem Specifika vzdělání a výchovy nadaného dítěte vypracovala samostatně a s použitím citovaných pramenů, literatury a dalších odborných zdrojů. V Praze dne 16. 4. 2012 podpis ……………………………. Anotace Tato práce podává v teoretické části informace o specifikách vzdělávání nadaných dětí v České republice. Obsahuje souhrn informací o historii, edukaci, přístupu i legislativě. Její praktická část je tvořena dotazníkem určeným široké veřejnosti a následně interpretuje jeho výsledky, názory a informovanost o nadaných dětech. Annotation The theoretical part of this thesis provides us about specifics of the education of gifted children in the Czech republic. Contains a summary of the history, education, legislation and access. The practical part of this thesis...
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Physikal Therapy for Vertebrogenic Pain Syndrome
Müllerová, Vladimíra ; Šlapáková, Kateřina (advisor) ; Tesařová, Martina (referee)
This thesis is focused on problems connected with vertebrogenic syndromes. General part consists of these subparts: diagnosis, occurrence, etiology, anatomy, pathogenesis and clinical manifestation of the disease, processes of diagnostic and therapy. Special part comprises these subparts: comprehensive medical rehabilitation, kinesiotherapy, physical therapy, occupational therapy, psychological and social aspects of disease. Final part of the thesis deals with case study
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Work motivation of dental hygienists
Dobrá, Eva ; Tesařová, Martina (advisor) ; Tichá, Michaela (referee)
This work is focused on the fluctuating motivation of dental hygienists. The goal of this work is to find the causes which lead to these dental hygienists to leave this career path, their motivation and demotivation inside their workplace. The theoretical part begins with defining the dental hygienist and describes her work routine and duties. Following part will be devoted to the fluctuation and turnover rate in the workforce, the causes and consequences related to it. The third section of the theoretical part will concentrate on motivation; motivation as a concept, personal fulfilment and financial compensation as a motivation. The empirical part analyzes the motivation of the participating sample group used for this study. Qualitative research methods were used; semi-structured interviews were conducted and then analyzed. The practical output is a description of the motivators and dissatisfactions of the selected test group of dental hygienists. With the help of the interviews, the conclusion to the motivations was reached and recommendations were assembled for dentists, managers and business owners of establishments which use the services of dental hygienists. Key words: dental hygienist, motivation, turnover, job satisfaction.
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The analysis of marketing communication of the International Film Festival Karlovy Vary in the year 2008
Tesařová, Michaela ; Šobr, Michal (advisor) ; Novotný, David Jan (referee)
My bachelor thesis deals with marketing communication of the International film festival Karlovy Vary. First of all I describe the International film festival and his history and the visitors who are typical for this festival. This film festival has specific advertisement and promotion. Film festival is also a part of art and film marketing. So I describe both parts of marketing in brief. Main theme of my bachelor thesis is the analysis of marketing communication of the International film festival in the year 2008. The analysis is based on theory which includes marketing mix. In the end of my thesis I assess level of promotion of the film festival.
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Human F1Fo-ATPsynthase deficiency
Suldovská, Sabina ; Tesařová, Markéta (advisor) ; Černá, Leona (referee)
F1FO-ATPsynthase is a key enzyme in energy metabolism of the cell. Its deficit is caused usually by mutations in two structural genes MT-ATP6 and MT-ATP8 encoded by the mitochondrial DNA or in nuclear genes ATPAF2 and TMEM70 encoding the biogenesis factors and structural gene ATP5E. Deficiency of the F1FO-ATPsynthase leads to progressive and serious phenotype affecting organs with high energy demands. The first symptoms usually occurs in neonatal age and prognosis of the disease is fatal. Mutations in these genes result in both qualitative and quantitative defects of the F1FO-ATPsynthase. The study of molecular bases of mitochondrial disorders including F1FO-ATPsynthase deficiency uses large number of biochemical and molecular-genetic methods to determine a proper diagnosis which is essential for the symptomatic therapy and genetic counselling in affected families. The aim of the diploma thesis was to characterise the F1FO-ATPsynthase deficiency in isolated mitochondria from the lines of cultured cells by the determination oligomycin- sensitive ATP-hydrolytic activity of the F1FO-ATPsynthase, enzymatic activities of the respiratory chain complexes and to analyse changes in the steady-state levels of the representative subunits and whole complex of the F1FO-ATPsynthase in comparison with controls. 3...
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Ankylosing Spondylitis and its Treatment Options in Terms of Physiotherapy
Dobrovolská, Zuzana ; Šlapáková, Kateřina (advisor) ; Tesařová, Martina (referee)
My bachelor thesis is focused on the disease called Bekhterev's disease, also known as ankylosing spondylitis. In the introduction to this thesis, I focused on my motivation and objective of this work, why I decided to write about this topic. I also mentioned the origin of this disease, its history and it's mechanism of occurence . In the practical part, I focused on the anatomical description of the human body, especially the back muscles, spine and root joints. There are even a detailed examination and diagnosis of the disease. I wanted to point out how the disease is incurable, how it occurs and whether the problems that entails can at least be partially be alleviated. I wanted to especially point out the methods of physical therapy which can be used to reduce progression of bekhterev's disease, teach affected people to live with this disease and be able to fit into the activities of daily life and ultimately find a job that is suitable for patients. I also wanted to compare two completely different stages of the disease: the patients with the fifth degree of disability with permanent movement restriction, and patients who have first degree of disability, which causes far more problems and of course, their individual approach to the disability and mental condition, both of which are very different. I...
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