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Recombinant preparation of TEAD transcription factor.
Lišková, Růžena ; Novák, Petr (advisor) ; Staněk, Ondřej (referee)
Recombinant preparation of TEAD transcription factor (abstract) The TEAD family transcription factors play an important role during devolopment of organisms, where their main purpose is to regulate organ size by activating expression of proteins involved in cell growth and differentiation and apoptosis inhibition. TEAD proteins activity is regulated by signalling pathways and interactions with coactivators. Disregulation of these mechanisms can lead to development of tumors, which is the reason why TEAD proteins became an interesting target for development of new anticancer drugs based on inhibiting their activity. There are several possibilities how to inhibit activity of a transcription factor including blocking its bond to DNA. To design a new drug that blocks transcription factors binding to DNA the structural basis of interaction of these two molecules has to be known first. In this thesis the DNA binding domain of human protein TEAD1 was prepared using the technique of recombinant expression in bacteria E. coli. Suitable conditions of protein production were found and the DNA binding domain of TEAD1 protein was purified so it will be possible to use it for structural analysis of its intraction with DNA.
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Influence of military activities on vegetation diversity in Doksy region
Staněk, Ondřej ; Vojta, Jaroslav (advisor) ; Volařík, Daniel (referee)
The thesis focuses on description of specific area of Doksy region, situated in northern Bohemia. This region was affected by fifty years of military management. Vegetation composition, species richness and several environmental characteristics were measured and statistically tested to determine the effect of military area on landscape and vegetation. No significant difference in species richness, but important changes in species composition were discovered. Also the environmental (soil) characteristics of the area were probably changed by the military history of landscape. There were no major changes in Beta diversity uncovered, probably because of different management leading to the same resulting changes applied in the landscape. Also four invasive plant species were found in high numbers present in the area connected with past military land-use.
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Centralized multirobot system
Staněk, Ondřej ; Obdržálek, David (advisor) ; Mráz, František (referee)
Title: Centralized multirobot system Author: Ondřej Staněk Department: The Department of Software Engineering Supervisor: RNDr. David Obdržálek Supervisor's e-mail address: David.Obdrzalek@mff.cuni.cz This thesis focuses on design and implementation of a centralized multi-robot system. A host computer (or cellphone) controls several tiny PocketBot2 mobile robots. These robots feature various sensors. They can perform line-following and they are equipped with a system for detecting obstacles and other robots. Although the PocketBot2 robots were designed and built within the frame of this thesis, the core of the thesis itself lies in the software. For the PocketBot2 hardware, an embedded control system was designed and implemented. It interfaces robot's sensors and carries out basic movement commands. Bluetooth technology is used for wireless data transfer between robots and the host. In the host, a multi-platform control library was implemented. It provides access to sensors of individual robots and controls their movement. The library ensures convenient interface for implementing centralized multi-robot algorithms. Keywords: robot, library, PocketBot, Bluetooth, Java
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Construction and characterization of recombinant adenylate cyclase toxoid of bacterium Bordetella pertussis carrying mycobacterial antigen TB7.7
Mikulecký, Pavel ; Vopálenský, Václav (referee) ; Staněk, Ondřej (advisor)
Bacterium Mycobacterium tuberculosis is an etiological agent of a deadly disease called tuberculosis that presents a global problem. According to The World Health Organization there are more than 2 billions people infected with latent tuberculosis all over the world. There is still need of specific, sensitive, quick and economic available method for identification of infected individuals. Currently in vitro blood tests are considered to be the best way of diagnosis. They are based on restimulation of specific T lymphocytes by mycobacterial antigens derived from virulent strains. There are several different approaches for enhancing of direct antigen delivery into antigen presenting cells and promising one is a genetically detoxified adenylate cyclase toxin (CyaA) of bacteria Bordetella pertussis. The main aim of the thesis includes construction and subsequent characterization of biological properties of CyaA protein carrying specific mycobacterial antigen TB7.7 in translocating domain. Here is shown that fusion protein CyaA-TB7.7 can form cation selective pores in target cell membranes and is able to deliver antigens into the cytosol of APC to be presented on surface with molecules MHC class II. Genetically detoxified CyaA- TB7.7 protein will be used to supplement current approaches such as also in vitro...
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Historical and present anthropogenic gradients in landscape
Staněk, Ondřej ; Klinerová, Tereza (referee) ; Vojta, Jaroslav (advisor)
My bachelor thesis is concerned in determining and classification of main anthropogenic influences in various ecosystems. Intensity and direction of landscape transformations such as nutrient income, terrain changes, disturbance, biomass removal or fragmentation is discussed and evaluated. Other landscape factors taking part in ecosystem endurance like different structure and pH of soils or various microclimatic conditions are also taken in notice. It seems that intensity of human influence varies depending on it's nature and it is difficult to propose a linear scale of these changes. From this point of view German hemeroby concept is discussed as possibly suitable scale of human influence. Historical and present anthropogenic gradients in landscape - Abstract (EN) Ondřej Staněk, 2010
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Visual Programming Backend for a Mobile Robot
Staněk, Ondřej ; Obdržálek, David (advisor) ; Bednárek, David (referee)
Title: Visual Programming Backend for a Mobile Robot Author: Bc. Ondřej Staněk Department: The Department of Software Engineering Supervisor: RNDr. David Obdržálek, Ph.D. Supervisor's e-mail address: David.Obdrzalek@mff.cuni.cz Abstract: In this work, the author designs and implements a solution for programming small mobile robots using a visual programming language. A suitable visual programming front-end is selected and back-end layers are created that allow execution of the program in a mobile robot. The author designs and implements a virtual machine that runs alongside the original robot firmware on an 8-bit microcontroller with limited resources. A code generator layer compiles the visual representation of the program into a sequence of bytecode instructions that is interpreted on board of the mobile robot. The solution supports typical features of procedural programming languages, in particular: variables, expressions, conditional statements, loops, static arrays, function calls and recursion. The emphasis is put on robustness of the implementation. To verify and maintain code quality, methods of automated software testing are used. Keywords: visual programming language, virtual machine, mobile robot, Blockly Powered by TCPDF (www.tcpdf.org)
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Desktop Game "Dáma"
Staněk, Ondřej ; Chmelař, Petr (referee) ; Kolář, Dušan (advisor)
This bachelor's thesis implements the draughts desktop game. Application allows to play according to different rules. It supports different ways of possible turns search of the player. One of them is the parallel computation.
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Evaluation of Bank Client's Creditworthiness
Staněk, Ondřej ; Čáp, Daniel (referee) ; Sághy Estélyi, Kristína (advisor)
The aim of this thesis is to evaluate the bank client´s creditworthiness for the purpose of loan financing and to formulate some proposals for its improvement. Some methods of financial and non-financial company analysis applied in banking practice are shown there. The proposals to ensure debt service are formulated on the basis of the results of analyzes.
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