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Rh - system.
Masopustová, Zdeňka ; Řeháček, Vít (advisor) ; Jiroušová, Hana (referee)
This work is focused on antigens and antibodies of the Rh system. Antibodies of the Rh system are not natural; they occur mostly as immune antibodies. Antigens of the Rh system are among the strongest immunogens with the greatest frequency. Antibody positivity was observed over a period of 5 years (2005 - 2009). Approximately 3500 blood samples per year were examined in screening tests and approximately 15 kinds of different antibodies with different occurrence frequency were identified. In some cases identification required more complicated procedures. The foundation of this work was acquired at the Transfusion and Haematology Ward at Kroměříž Hospital.
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Cryoconservation of erythrocytes and its use in civilian and military healthcare
Sládková, Eliška ; Řeháček, Vít (advisor) ; Jiroušová, Hana (referee)
The theme of my final paper is Cryoconservation of erythrocytes and its use in civilian and military healthcare. This work consist of theoretical part and practical part. The first part of the thesis introduces cryobiology basics, types of cryoprotectants and the cryopreservation of erythrocytes itself. The thesis describes individual procedural steps leading to the final transfusion product, i.e. methods of blood collection, blood's pre- glycerolisation processing, glycerolisation itself and freezing. Furthermore, it describes issues associated with storage of frozen blood products, as well as with their thawing and deglycerolisation. The second part, which is practical, deals with quality control of cryopreserved erythrocytes. It describes various quality parameters and acquaints readers with the results of such measured parameters.
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Testing of HLA antigens and antibodies in the laboratory of transfusion department.
Hájková, Lenka ; Řeháček, Vít (advisor) ; Jílek, Petr (referee)
Author: Lenka Hájková Title: Testing of HLA antigens and antibodies in the laboratory of transfusion department Bachelor's Thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Field of study: Medical Laboratory Technician Background: Retrospective study, overview of investigations of potential kidney recipients from 12 participating hemodialysis centres during 3 years (2008 - 2010). Methods: Following examinations were performed on potential kidney recipients in the HLA laboratory of the Transfusion Department, University Hospital Hradec Králové: serological fenotyping HLA-A, B (CDC test), PCR HLA-DRB1* genotyping (SSO, SSP) and screening of antileukocyte antibodies (CDC test). Results: The total number of 154 potential kidney recipients were examined during the period of time from 1. 1. 2008 to 31. 12. 2010 (2008: 57, 2009: 46, 2010: 51). Screening of antileukocyte antibodies was performed on 1 140 samples, the most frequent result of antibody levels was between 0 - 30 %. Most patiens were at age of 41 - 61 years, male/female ratio was 2:1. The most frequent HLA antigens concerning the group of patients with kidney diseases were HLA-A2, HLA-B8 and HLA-DRB1*11. Conclusions: During the period under consideration the number of newly registered potential kidney recipients did not...
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Kidney Transplantation: Donor-Recipient Pairing in University Hospital Hradec Králové
Moravcová, Lucie ; Řeháček, Vít (advisor) ; Jílek, Petr (referee)
8 ABSTRACT Author: Bc. Lucie Moravcová Supervisor: MUDr. Vít Řeháček Charles University, Faculty of Pharmacy in Hradec Králové Title of master's thesis: Kidney transplantation: donor-recipient pairing in University Hospital Hradec Králové Background: The aim of this study was to determine HLA, blood group, age and sex match in donor-recipient pairing in kidney transplantation. HLA alleles of deceased donors were typed in Transfusion Department of the University Hospital Hradec Králové. Methods: Donor's HLA-A*, HLA-B* and HLA-DRB1* alleles were typed by PCR - SSP method. Complex data evaluating and processing was then performed. Results: 97 deceased donors were tested between 2013 and 2018. A total of 98 kidneys received from them were subsequently transplanted to 98 recipients in University Hospital Hradec Králové. 60,2 % of the donors were men, 63,3 % of the recipients were men. Most of the donors, as well as the recipients, were 51-70 years old (50,0 % and 59,2 %, respectively). The most common diagnoses in the group of deceased donors were associated with brain damage (66,3 %), the most common cause of renal failure in the group of recipients was chronic inflammatory kidney disease (41,8 %). All 98 transplantations (100,0 %) were AB0 compatible. 74 transplantations (75,5 %) were RhD compatible. 5...
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RhD antigen screening by molecular genetic methods
Bakerová, Dagmar ; Řeháček, Vít (advisor) ; Jílek, Petr (referee)
Author: Bc. Dagmar Bakerová Supervisor: MUDr. Vít Řeháček Charles University, Faculty of Pharmacy in Hradec Králové Title of diploma thesis: RhD antigen screening by molecular genetic methods This thesis deals with the genotyping of weak and partial antigens using molecular genetic methods. The main aim is to evaluate the rate of the representation of individual types of variant and weak RhD antigens in first-time blood donors, patients and pregnant women. Testing took place at the Transfusion Department of University Hospital Hradec Králové between October 2015 and February 2019. The PCR-SSP method was used for RHD genotyping using commercially supplied BAGene SSP kits from BAGene Health Care. The study includes 32 samples from first-time blood donors in the reference period to determine the specific type of RhD antigen, and 188 samples from patients and pregnant women, for whom serological methods could not be used to unequivocally identify the RhD antigen. For all pregnant women, moreover, the genotyping result was a factor in determining whether to administer anti-D immunoglobulin. This RHD genotyping for all serologically ambiguous samples has made it possible to determine a specific type of partial or weak RhD antigen. In the donor group, the weak RhD antigen was detected in 1.12 % of cases...
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The Frequency of HLA Antigens in the Group of Plasma Donors of the Transfusion Department, University Hospital, Hradec Králové
Nováková, Petra ; Řeháček, Vít (advisor) ; Jílek, Petr (referee)
Author: Petra Novakova Title: The frequency of HLA antigens in the group of plasma donor of the Transfusion Departmen, University Hospital, Hradec Králové Bachelor thesis Charles University, Faculty of Pharmacy in Hradec Kralove Field of study: Medical Laboratory Technician Background: The major objective of this study is the statistical data processing in order to determine the frequency of HLA antigens in the group of plasma donors. Methods: The serological typing of HLA antigens using the lymphocytotoxic test was performed among plasma donors of the Transfusion Department of the University Hospital Hradec Kralove. On a sample group of 200 plasma donors was determined the frequency of HLA antigens by blood group and gender on the basis of a serological examination. The collected data of the observed sample of aforementioned group were processed using Microsoft Excel in relation to the gender and blood group of the donor. Results: It was discovered that within the observed sample there is the highest frequency of HLA-A antigens 2, 1 and 3; HLA-B antigens 7, 44, 35 and HLA-C antigens 7, 6, 4. The most frequented combination of HLA antigens within the observed sample of 200 plasma donors regardless blood group and gender are combinations of HLA-A 1,2 (15x) HLA-A 2,3 (15x), HLA-B 8,44 (8x), HLA-B 7,...
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Frequency of HLA Antigens in Patients before Bone Marrow Transplantation
Moravcová, Lucie ; Řeháček, Vít (advisor) ; Jílek, Petr (referee)
Author: Lucie Moravcová Title: Frequency of HLA Antigens in Patients before Bone Marrow Transplantation Bachelor's Thesis Charles University, Faculty of Pharmacy in Hradec Kralove Field of study: Medical Laboratory Technician Background: Elaborated overview of frequency of HLA antigens in patients before bone marrow transplantation who were examined from 2014 to 2016 in Transfusion Department of the University Hospital Hradec Kralove. Compare our data with data provided by Czech National Marrow Donors Registry. Methods: Patient's HLA alleles were typed by PCR - SSP method. Family members were typed by serological method. After that was conducted a complex statistic processing of our data. Results: 154 patients before bone marrow transplantation and 157 of their family members were examined from 2014 to 2016. There were 85 men and 69 women tested among the patients. Most patients were between 61 - 70 years of age. Acute myeloid leukaemia was the most common diagnose. A family study was conducted for 92 patients. 36 potential suitable donors were found among family members. No allele had significantly high frequency in the HLA-A loci of patients. In the HLA-B loci had significantly high frequency HLA-B*15 allele (10,065 % vs. 6,800 %). HLA-DRB1*01 allele was also more frequent (13,961 % vs. 9,897 %)....
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Comparison phenotype with genotype of red blood cells antigens using serologic and microarray techniques
Králová, Jana ; Řeháček, Vít (advisor) ; Rathouská, Jana (referee)
The diploma thesis deals with the erythrocyte antigens genotyping using microarray techniques. It compares the results of microarray methods with the phenotypes obtained by serological techniques. The aim of the theses is to evaluate the degree of identical results (phenotype - genotype) and the applicability of microarray techniques in the immunohematology laboratory. The testing was carried out in the National Reference Laboratory, Institute of Hematology and Blood Transfusion, Prague, in the period January 2014 - December 2015. Methods BLOODchip Reference and ID CORE XT™, Progenika Biopharma S. A., were used for genotyping. Serological determination of phenotypes was performed using Grifols and BioRad gel agglutination techniques. The study encompassed 78 samples of healthy donors of diagnostic red blood cells, 62 patient samples with no possibility to determine the presence of certain antigens by serological methods, and 28 patient samples with the discrepant result of RhD determination. Genotyping of the donors' samples enabled to record variant and weak antigens not detectable by serological methods. Donor samples displayed 100% conformity of serological and genotyping results. Considerable variability in patients' samples was found in comparism of genotyping and serological techniques. The...
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