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Diagnosis of leishmaniases in humans and dogs
Černá, Eva ; Spitzová, Tatiana (advisor) ; Votýpka, Jan (referee)
Leishmaniasis is a serious disease caused by parasites that affects both people and animals. In people, this disease has three forms, cutaneous, mucocutaneous and visceral form. Visceral form is lethal if it's left untreated. Leismaniasis is usually diagnosed by using regular parasitological methods based on histocytological analysis. In the last few years, there has been a considerable progress in serological and molecular diagnostics. This thesis focuses on description and comparison of the traditional and the new diagnostic methods. Attention is paid primarily to the new methods, therefore serological and molecular. These methods are compared by their sensitivity, specifity, field application potential, financial costs and time consumption. Both human and canine diagnostics are mentioned due to the fact that dogs are a significant reservoir. Key words leishmaniasis, diagnostics, parasitology, humans, dogs, method, molecular, serological, sensitivity, specifity
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Native hyaluronan as a delivery system for hydrophobic drugs
Černá, Eva ; Mravec, Filip (referee) ; Pekař, Miloslav (advisor)
The aim of this paper is to discover whether it is possible to use the native form of hyaluronic acid as a hydrophobic drug carrier for a targeted distribution in the body. In its structure, hyaluronic acid is a linear high molecular weight biopolysaccharide which is found in most living organisms. Hyaluronan is involved in many physiological processes and therefore is essential for the functionality of the human body. It is in most tissues of the human body, high concentration is in the skin, the vitreous body and is also observed in cancer cells that contain several receptors for hyaluronan. These receptors include CD44 and RHAMM. The interaction of the hyaluronic acid delivery system and the hydrophobic medicinal with these receptors could ensure a free passage for drugs to the affected tissue, where the release of the drug would destroy the affected cells. The drug would directly target the damaged tissue and did not burden the rest of the body like the cytotoxic agents do. In this paper the native form of hyaluronic acid, which we normally find in the human organism, was chosen as the carrier. Its properties do not stand above other carrier systems, but its biocompatibility and biodegradability in the body greatly exceed them. High molecular weight hyaluronic acid was used as a carrier and the hydrophobic dye sudan red G, a substance of similar properties, was used instead of a hydrophobic drug.
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Verification of the composition of the concrete durability of cement-concrete covers
Černá, Eva ; Ťažký, Tomáš (referee) ; Hela, Rudolf (advisor)
Alcali – silica reaction often causes destruction of a concrete.constructions. In the theoretical part is described creation and causes of the ASR including assessment of individual components of concrete and their testing. The practical part is devoted to the results of some control tests of the concrete cover in the section 18 of the highway D1 (Měřín - Velké Meziříčí) and section D1-20 (Velké Meziříčí - Lhotka), the mechanical properties of the upper and the lower layer of the concrete cover and measuring the resistance of concrete against chemical deicing agents.
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Surrealistic Moments in Raymond Queneau's Novels
Černá, Eva ; Jamek, Václav (advisor) ; Voldřichová - Beránková, Eva (referee)
Surrealistic Moments in Raymond Queneau's Novels The objective of this thesis is to study the connection between Raymond Queneau's novels and surrealism. Raymond Queneau was part of the surrealistic group for only a relatively short time and after he left it, he fully rejected the surrealistic movement and concentrated his literary output in another direction. Nevertheless, there are surrealistic moments to be found in his work, on which this thesis will focus. The thesis contains a brief characterisation of surrealism as a literary movement and a basic overview of Raymond Queneau's life and the structure of his works. Special focus is given to the part of the author's life when he was active in the surrealistic group. The thesis further studies the period of time when Raymond Queneau was no longer part of the surrealistic group and when he turned his attention to completely different literary aspects of his production and participated in the work of other literary groups, whose methods differed substantially from the methods of surrealism (Oulipo); special focus is given to the significant aspects of the author's output, i.e. emphasis on the literary form, structure and order of his works. The main focus of the thesis is to study the surrealistic moments in Raymond Queneau's novels, which occur in his...
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Medieval Glass in Prague
Prokopová, Anna ; Klápště, Jan (advisor) ; Černá, Eva (referee)
This work considers finds of medieval glass in the historic centre of Prague on the basis of archaeological material from the National Herritage Institute excavations. Besides the typology of glass vessels, special attention is paid to the concerning the social level of the society of that time, resp. the study of glass imports that can inform us of the commercial or cultural contacts of the given locality. Especially the enameled glass play an important role in studies of the extraordinary social and economic status. The archaeological evidences of medieval glass has been found in Prague since the end of the 19 th century. The end of the sixtieths brought large finds of medieval glass fragments which most has not been studied as yet. Some of them are included into this work, which is aimed at the region of Old and New Town, whereas the finds of Prague Castle has been left aside. In the study of the popularity of glass tableware, it is possible to observe the process well documented in the majority of Czech towns. In Prague glass ware fragments appeared quite early, already in the 12th century and also in the following century glass presented luxury ware intended for the highest social level. Towards the end of the 13th century the situation began to change. During the first half of the 14th century we...
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Hodnocení barvy medu
Černá, Eva
The aim of the thesis on "Evaluation of the color of honey" was to determine why the instrument can not Lovibond Honey Colorpod 440110 to evaluate color of all types of honey and compare results of with spectrophotometric measurement methods. The work is divided into theoretical and practical part. The theoretical part is dedicated to honey, its composition and methods of evaluating color. The practical part describes the results obtained instrument Lovibond Honey Colorpod -- 440110, spectrophotometers Konica Minolta -- 3500d a FT -- NIR Antaris. In UV/VIS region not been demonstrated reason of errors of photometer. Maximum lightness had a very light honeys and the largest share of yellow had light honey. The highest values of absorbance attain dark honeys. In color space CIE L* a* b* had the largest share of red. Evaluation of the color of honey in the UV/VIS can not prove adulteration of honey always unambiguously, but there are signs by which honey can be described as suspicious. By analyzing the NIR spectrum is falsification of honey demonstrated using discriminant analysis.
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