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Presence and function of glycosidas in the reproductive tract of mammals
Slavík, Martin ; Postlerová, Pavla (advisor) ; Tuzhilkin, Roman (referee)
Glycosidases are enzymes that hydrolytically cleave polysaccharide structures and have fundamental importance above all for processing the glycocalyx on the surface of the sperm during the fertilization process, so that the sperm can finally successfully interact with the zona pellucida and fuse with the oocyte membrane. The bachelor thesis investigates the activities of several important glycosidases (β-galactosidases, α-galactosidases, α-mannosidases and neuraminidases) in mixed samples of reproductive fluids, specifically it is epididymal fluid, seminal plasma and oviductal fluid, originating from the domestic pig. Another sample in which the activity was measured of the aforementioned enzymes was ejaculated sperm. The activities of the given enzymes were determined using fluorescent substrates in slightly acidic and neutral pH. In addition to determining specific activity of individual enzymes, this bachelor's thesis was enriched with qualitative information regarding glycoproteins in male and female reproductive fluids and ejaculated sperm lysate. Glycoproteins are the dominant component of the sperm glycocalyx and many of them also represent the corresponding naturally occurring substrates for the above-mentioned glycosidases. Based on electrophoretic separation followed by staining, the...
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Use of uranine-probe method to determine evaporation rate from porous media
Pohan, Tomáš ; Slavík, Martin (advisor) ; Mareš, Jakub (referee)
Evaporation from porous media affects many natural processes. In my work, I focused on the second evaporation phase, calculating the evaporation rate using Fick's law based on air temperature and air humidity above the surface, the water-vapor permeability of the porous material, and the evaporation front depth below the surface. To determine this depth, I used the uranine-probe method using sodium fluorescein. The work aimed to calculate the evaporation rate using Fick's law and values of the evaporation front depth determined by the uranine-probe, for 6 sandy soil samples and 3 types of rocks. A sub-goal was to verify the applicability of the uranine-probe for determining the depth of the evaporation front in sandy soil and in 12 different rock samples. Results show successful determination of evaporation front depth for both sand and rocks. The uranine-probe method's accuracy averaged 2.0 mm for sand. The difference in the values determined by the uranine-probe method compared to another, independent method (detection of the evaporation front from the color change of the fluorescein solution) in the sand was on average 1.7 mm, but I did not study this for the rock samples. All evaporation experiments confirmed that the evaporation rate, both measured and calculated, decrease with increasing depth...
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Contaminant transport in karst environment and tracer tests in karst system near Turnov
Malechová, Kateřina ; Slavík, Martin (advisor) ; Weiss, Tomáš (referee)
Karst areas are an important source of drinking water but they are at significant risk of contamination. The theoretical part of the thesis focuses on different types of contaminants, their sources and their ways of transport in karst areas. Due to the high velocity of water flow in karst areas, most contaminants are transported rapidly and spread over long distances. Therefore, it is necessary to actively address the issue of contamination in karst environments, focusing mainly on contamination prevention and water flow studies. In the practical part of the thesis, a quantitative tracer test was carried out in the karst system near Turnov using NaCl as a tracer. The measurements confirmed a high water flow velocity of several kilometers per day and the dominance of advection as the main transport process. These results are consistent with previous research. The connection between the sinking stream Šálení smyslů and the Slapy and Bezednice springs was also reconfirmed, with a total recovery of around 50%. This implies that water from the Šálení smyslů most likely flows to other, yet unknown, locations. No communication with the Bartošova pec cave or with the springs used as a source for the local brewery has been demonstrated, so the question remains where all the water from the sinking stream...
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Measuring the retention curve of rocks: the example of sandstone from the Jizera formation
Fataciune, Sotir ; Slavík, Martin (advisor) ; Ondovčin, Tomáš (referee)
Retention curve describes the ability of an unsaturated porous medium to retain water at different suction pressures. It thus plays an important role in providing information on the movement and occurrence of water in the vadose zone. While the determination of the retention curve for soil is common, it is far less common in the case of rocks. The aim of this bachelor thesis is to determine the retention curve of sandstone from the Jestrebicka jehla in Kokorinsko using the tensiometer - weight method. A partial aim is to compare this curve with two previous measurements on the same sandstone: i) measurements by student A. Studencová, using the tensiometer - weight method, and ii) measurements made by the laboratory of Research Institute for Soil and Water Conservation, according to a slightly modified method of CSN ISO 11274. There were notable differences between the retention curves. The retention curve according to the CSN for a given suction pressure showed lower moisture values, which is probably due to insufficient saturation of the sample and air entrapment in the rock pores. Relatively large differences in the retention curve between two independent tensiometer - weight method measurements were also found, which are probably due to insufficient contact between the tensiometer and the porous...
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Advanced Segmentation of a Simulated Network
Slávik, Mark ; Komosný, Dan (referee) ; Benedikt, Jan (advisor)
The diploma thesis deals with the simulation of available routing protocols in the NS3 simulator and in the Quagga module. The functions of simulated routing protocols are described in the theoretical part. In the practical part, the functionality of the NS3 simulator and the RIP, OLSR and OSPF, BGP routing protocols from the Quagga module are introduced using a simple topology. Finally, the network is extended and several subnets are added. Using available analysis tools, the network is subsequently analyzed and evaluated with different scenarios.
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Interface for Communication on Hardware Accelerated Circuits
Slávik, Mark ; Cíbik, Peter (referee) ; Smékal, David (advisor)
The work deals with the description and implementation of the MicroSD interface on programmable logic arrays. The thesis describes the FPGA theory, VHDL language, Vivado environment,pheripherals on FPGA board, VitisHLS. Next, the implementation of the code and its simulation is described. At the end, digital image processing using FPGA and Micro SD card is explained.
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PLC controlled system for manipulated arm
Slavík, Miroslav ; Zemánek, Pavel (referee) ; Pásek, Jan (advisor)
In this bachelor´s thesis is solved automatic task of PLC controled manipulated arm. For this controled is used Siemens Simatic 200 with programing enviroment MicroWin. The servos are moving with parts of arm. The servos are conected to the PLC trought PCB of electronics interface. PCB is created specially for this aim. Design of PCB is described. Details description is PLC´s program. Comunication between PLC and SCADA is trought a PPI cable. Vizualition of proces is runing on the ControlWeb by Moravians instrument, this is descripted also. To the Manipulated arm is instaled conveyor. Boxes are transporting by conveyor. Pyramid is build from boxes.
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Analysis of pitch perception depending on the spectrum
Slávik, Martin ; Indrák, Michal (referee) ; Jirásek, Ondřej (advisor)
This thesis is dealing with the research of the influence of individual harmonic components on the perception of sound height. As a part of the work has been made an internet questionnaire on a web address www.analyzavyšek.9e.cz, where individual respondents completed listening test compound of 46 samples. This experiment is based on semantic differential method. Individual samples are compound of sine wave – tone and examined sample, which respondents evaluates on three bipolar scales. The samples were either modelled by additive synthesis or modified sampled sounds of instruments. Individual samples were divided to four major groups and other minor groups for clarity. The purpose was to find which parts, combinations and harmonic components of sound and tone can affect perception of tone height.
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The Study of Production's Operation Management
Slavík, Martin ; Škaroupka, Jan (referee) ; Jurová, Marie (advisor)
In my diploma thesis I have focused on the optimization of ‘Production Operational Management’ in terms of change management and work organization in the department of thermo case and pre-production within the company Automotive Lighting, s.r.o. which is a producer of automobile light technique. The intention of the first part is to produce general characteristics of the company. The analysis of the current stage results from the production system and the organizational structure particularly in the pre-production department of thermo cases. Based on this analysis I have designed the proposal so that it consists of a new organizational structure and a new work organization with a view to customer satisfaction within a demanding product while taking into consideration quality and time. In the final part of this thesis I have looked at the contribution and feasibility conditions of the output proposal.
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Measurement of Stream Flow Rate Using Salt Dilution Method
Burdych, Marek ; Ondovčin, Tomáš (advisor) ; Slavík, Martin (referee)
Tracer dilution method for measuring flow rate in surface streams is the main topic of the bachelor's thesis. The first part contains a summary of the most important flow measuring techniques for surface streams, including current meter, float method, FlowTracker, ADCP, volumetric method, and computing techniques based on channel cross-section data. Large part is dedicated to tracer dilution method. The findings of field measurements of flow made on the Lo- děnice River using the salt dilution approach are detailed in the second section. Five conductometers were used to measure the water conductivity at three di- fferent sites. Conductometry was performed simultaneously in two different distances from injection site. Throughout the experiment, a sixth conductome- ter recorded the background conductivity value as a standard. The predicted flow values are compared to those acquired from nearby hyd- rometric station of the Czech Hydrometeorological Institute (CHMI) and from observations made using Flowtracker. Diferences in measured flow rate were interpreted as tracer losses. These losses were estimated. Keywords: hydrology, surface water, water stream, tracer dilution, conducti- vity, tracer
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