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Spoil heap toxicity and possibilities for remediation
Venclovská, Lenka ; Frouz, Jan (advisor) ; Lukavský, Jaromír (referee)
The content of this diploma thesis is to verify of possibilities for remediation spoil heap toxicity in Sokolov area, by the help of creating biological crusts. The main question of this work is the possibility of using algae to create biological crust on toxical substrates, which could improve the properties of substrates and contribute to the acceleration of succession. The correctness of this theory was tested using two tests on toxic soils in spoil heaps near Sokolov. In the first test (laboratory test) have been chosen two of the soils and the higher plants, in most cases, even did not germinate. That is why we decided to verify the second option to create biological crust in these phytotoxic soils. Therefore we applied selected kinds of algae in the field, some areas were liming, and the field has been monitoring and sampling during next three years. The results show that the application of dolomitic limestone does not affect progress of biological crusts. The result of the fluorescent microscopy clearly shows, that Klebsormidium algae was the only one of the tested algae, which survived and progressed compact visible crusts.
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Spoil heap toxicity and possibilities for remediation
Venclovská, Lenka ; Frouz, Jan (advisor) ; Lukavský, Jaromír (referee)
The content of this diploma thesis is to verify of possibilities for remediation spoil heap toxicity in Sokolov area, by the help of creating biological crusts. The main question of this work is the possibility of using algae to create biological crust on toxical substrates, which could improve the properties of substrates and contribute to the acceleration of succession. The correctness of this theory was tested using two tests on toxic soils in spoil heaps near Sokolov. In the first test (laboratory test) have been chosen two of the soils and the higher plants, in most cases, even did not germinate. That is why we decided to verify the second option to create biological crust in these phytotoxic soils. Therefore we applied selected kinds of algae in the field, some areas were liming, and the field has been monitoring and sampling during next three years. The results show that the application of dolomitic limestone does not affect progress of biological crusts. The result of the fluorescent microscopy clearly shows, that Klebsormidium algae was the only one of the tested algae, which survived and progressed compact visible crusts.
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Phytoplankton in experimental ponds: colonization and seasonal succession
Hrušková, Lenka ; Nedbalová, Linda (advisor) ; Lukavský, Jaromír (referee)
Artificial ponds are excellent for the study of phytoplankton ecology. They permit repeatable initial conditions and sufficient replication of independent experimental units in complex experiments to test hypotheses about the control of structure and function in natural communities. There were 20 experimental ponds constructed in Kokoř nsko Protected Landscape Area, from which samples were taken and analyzed. This study was part of the EU BIOPOOL. During the first two years after the filling of the ponds the colonization and the seasonal development of phytoplankton communities in the ponds were examined. The phytoplankton species composition was determined, then species richness and relative abundance of individual taxa were quantified. Species composition data were compared with the environmental parameters measured (temperature, oxygen concentration, pH, transparency, conductivity, depth, chlorophyll-a concentration, and zooplankton). In the spring 2007, the ponds were periodic, and followed a similar trend over time (February- April). There were dominated by the following taxonomic groups: Dinophyta, Chrysophyta, Cryptophyta, and Euglenophyta. After installing foil on the bottom of the ponds, the ponds were of permanent character. In terms of environmental parameters, the individual ponds...
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Mikroprocedura řasového testu
Lukavský, Jaromír
Growth algal bioassay is based on long-term cultivation of a testing alga with tested substance in nutrient solution, or in a sample from nature. The bioassay is at least 3 generations and also chronic affects or influence of repruction process can be so detected. The yield of biomass of testing alga in a sample from nature represent the maximal biomass which could be expected in nature without limitation with light, temperature, CO2 and predation.Miniaturized bioassay is exploating cultivation of algae in wells of an immunological plate in volumes of 0.2 ml. In a plate 9x13 cm, there are 96 wells which enables us to expose 10 samples every in 6 replicates, 2x25 plates in a cultivation unit of size of a common table has capacity of 3 000 miniaturized cultures. Their filling, inoculation and evaluation can be facilitated with a great spectrum of tools and equipments for immunological assays
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Řasová sbírka - banka tesovacích kmenů sinic a řas
Lukavský, Jaromír
Culture collections, including algal, facilitate experiments and teaching by a speedy delivery of determined and pure cultures of required strains. There are about 7 000 strains in ca 50 algal collection over the world. The most easy body of information about some strains is in world dbase of World Federation of Culture Collections of Microorganisms : http://wdcm.nig.ac.jp/
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