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Historie a technologie výroby pařených sýrů
Konečná, Markéta
This bachelor thesis is focusing on history and production technology of steamed cheese. In the beginning there is part about history of cheese making since the Middle Ages till nowadays. The next chapter describes composition of milk and the ingredients which have effect on the quality of steamed cheese. Main part of the thesis describes technological process of making of steamed cheese and possible defects which can occur. In the next part of the thesis range of goods of steamed cheese from The Czech Republic and other part of the world is listed. The thesis is finishing with quality parameters of different types of steamed cheese and the analytical methods which are used for determination of individual quality parameters.
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Vliv přerušení teplotního řetězce na mikroflóru mléčných výrobků
Konečná, Markéta
This thesis deals with the effect of temperature chain breaks on the microflora of dairy products. In the introduction, the technological process of all nine samples used for the analyses is described – Jadel bily, Jadel koreneny, Zerve, Svacinka, Maslo, Mozzarella, Tvaroh mekky, Bily jogurt, and Vanilkovy krem. The next part of the thesis is devoted to the microbiological requirements of each product. The last part of the theoretical work describes the microbiological risks of the samples. The thesis is concluded with the practical part. The samples were subjected to a breakage of temperature chain and then the effect of the temperature breakage was investigated. The total number of microorganisms, coliform bacteria, yeasts, and molds was determined in the work.
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Zkušenost zahraničích studentů: případová studie absolventů FRRMS Mendelu
Konečná, Jitka-Markéta
This diploma thesis deals with the experience of foreign students of the Faculty of Regional Development and International Studies at Mendel University in Brno. The theoretical part focuses on the meta concept of migration and excluded study migration, the internationalization of higher education, the effects of study migration and the experience of the migrat-ing student through acculturation. Survey of eleven graduates was conduct-ed by semi structured interviews. The survey evaluated their experience in three areas: study experience, acculturation experience and a postgraduate experience. The aim of this work is to describe the experience of foreign students, to draw attention to the challenges and obstacles to which foreign students are exposed and compile recommendations for improvements based on statements.
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Ecotoxicity of selected extinguishing agents
Konečná, Markéta ; Vávrová, Milada (referee) ; Zlámalová Gargošová, Helena (advisor)
The dynamic development of industry and the constant production of new substances affecting the environment is currently one of the priorities of the interests of the human population. This thesis is focused on the ecotoxicological evaluation of selected extinguishing agent which are applied in case of fire. They must effectively extinguish fire because a live protection and material resources in any case very important, but Theky should be also environmentally friendly. In this work were tested surfactants, which are the main component of foaming extinguishing agent with the following commercial names: STAMEX F-15, F-15 EXPYROL, MOUSOL APS F-15, FINIFLAM F-15 and PYROCOOL B. Since surfactants have a negative impact mainly on aquatic ecosystem assessment were mainly used ecotoxicity tests using aquatic organisms. Test organisms were aquatic crustacean Daphnia magna and Thamnocephalus platyurus aquatic dicotyledons plant Lemna minor and terrestrial monocotyledons plant Sinapis alba. Based on the results of the tests were values of LC50, EC50 and IC50 for the tested substances determined and their ecotoxicity compared.
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Extraction of nucleotides from plant matrix and their determination by ion-exchange high performance liquid chromatography
Konečná, Markéta ; Sobotníková, Jana (advisor) ; Coufal, Pavel (referee)
In this thesis chromatographic conditions for the HPLC analysis of ribonucleotides AMP, ADP and ATP were optimized. For their separation chromatographic column, Nucleogel SAX 1000-8, 50 x 4,6 mm, from the German company Marcherey-Nagel was used. The mobile phase of a 0,2 mol/L solution of KH2PO4, its pH was adjusted with 1mol/L with potassium hydroxide to pH = 5,00. As the most suitable flow rate was selected 1,0 mL/min. The analysis was conducted using an isocratic elution. Detection was conducted by a DAD detector at a wavelength of λ = 260 nm. Substances were eluted in the following order AMP (tr = 0,911 min), ADP (tr = 1,667 min) and ATP (tr = 7,262 min). The total analysis time of the mixture of standards, under the conditions mentioned above, lasted for 10 minutes. For the extraction of adenosine-5-ribonucloetides the real matrix frozen and lyophilized leaves of tobacco virginia (Nicotiana tabacum L.) have been used. Two methods of extraction were applied. The first procedure was based on the extraction of AMP, ADP and ATP from the leaves of the tobacco plant by boiling deionized water. In the second method 0,07 mol/L HClO4 for the extraction instead of deionized water was used. Keywords: HPLC, adenosine ribonucleotides, AMP, ADP, ATP, tobacco virginia
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Phytoextraction of Bromazepam
Konečná, Markéta ; Smrček, Stanislav (advisor) ; Petrová, Šárka (referee)
The aim of this bachelor thesis was to determine the possibility of phytoextraction of benzodiazepine bromazepam as a widely used drug with anxiolytic effects. Benzodiazepines are compounds with high biological activity and together with their metabolites are excreted into urine. These substances then get into municipal wastewater treatment plant, whereas efficiency of removal in wastewater treatment plants is not high and compounds enter into surface water. Afterwards, the entire ecosystem including food chains is contaminated. A long-term exposure to trace concentrations can have a great effect on aquatic and terrestrial organisms. Maize (Zea mays) was used to study the phytoextraction. The Murashige and Skoog medium was enriched by 10 mg · L-1 bromazepam and samples of medium were taken at 24 hour intervals. After that, samples were analysed in reverse phase at 233 nm by HPLC/UV. Time dependence of bromazepam concentrations in medium was evaluated, which showed decrease of ca. 40 % during 168 hours of cultivation. Ability of corn cultivar to extract bromazepam from aqueous solutions was clearly demonstrated. The amount of extracted bromazepam corresponds to 0.03 milligrams per 1 gram of plant fresh weight. Although the tested concentrations of the xenobiotic used are substantially higher than the actual...
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