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Fluorescenční kinetická mikroskopie: 2D zobrazování variabilní fluorescence chlorofylu jednotlivých buněk řas se spektrálním rozlišením
Prášil, Ondřej ; Šetlíková, Eva ; Veselka, D.
The fluorescence kinetic microscopy (FKM) provides new dimension in application of chlorophyll fluorescence techniques. It combines classical fluorescence microscopy with 2D kinetic detection systems. FKM allows to examine photosynthesis of individual algal cells or on the cellular level of macroscopic structures. FKM is well suited to study heterogeneity in photosynthetic response among individual cells in algal cultures or in natural mixed populations. Special microscopic chambers allow to maintain live cells under optimal conditions for several hours or days and to follow evolution of photosynthesis in individual cells during the course of their growth cycle. Recent technical developments have allowed to determine FKM fluorescence kinetic with spectral resolution
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Diamond growth on silicon and WC-Co by microwave plasma chemical vapor deposition
Frgala, Z. ; Kudrle, V. ; Janča, J. ; Buršíková, V. ; Vašina, P. ; Meško, M. ; Buršík, Jiří ; Kadlečíková, M. ; Klapetek, P.
We studied the growth of microcrystalline diamond films on pre-treated Si and WC-Co substrates by microwave plasma chemical vapor deposition.We observed the interesting time dependence of the negative bias voltage during nucleation stage which is useful for nucleation process monitoring. The layers were analysed by Raman spectroscopy, scanning electron microscopy and atomic force microscopy.
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