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Isolation and Characterization of Protoplasts and their Utilization for Model Membrane
Nováková, Kateřina ; Navrátil, Tomáš ; Šestáková, Ivana ; Langmaier, Jan ; Heyrovský, Michael ; Zámečníková, B. ; Vodičková, H.
Protoplasts are prepared form plant cells by removing their cell walls, which are usually destroyed mechanically or by enzymes. Protoplasts exhibit the spherical shape after removing of their cell walls. They can be isolated from a range of plant tissues: leaves, stems, roots, anthers, and even pollen. Plasmatic membranes surrounding protoplasts can be used as a source of model membranes. Therefore, some parts of plasmatic membranes or subcellular organelle membranes, which contain specific transport systems, can be isolated and integrated to model phospholipid membranes. State of such model membranes can be characterized by electrochemical impedance spectrometry (EIS) and the transported species can be determined with the use of voltammetric methods.
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