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The possibility of using molecular markers to identify the commodities subject to CITES
HYNKOVÁ, Veronika
This thesis is focused primarily on the possibilities of using molecular markers to identify the commodities subject to CITES. Illegal trade with these articles is common even in the Czech Republic. Overview the most used methods could facilitate the fight against this kind of crime. Molecular markers are ideal for the identification of species and other taxonomic units, because unlike the morphological identification does not require intact specimens. DNA can be extracted from highly processed and degraded products which are commonly found on the market.
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Optimalizace markerů pro aplikace rozšířené reality
Kabelka, Petr
This thesis is focused on the libraries and frameworks for augmented reality. Part of this work is empirical testing for evaluation and comparison of the libraries with each other. The rest of the work is optimization of the markers for detection. The results are compared with previous values.
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Fylogeography and genetics variability of \kur{Diuraphis noxia} (\kur{Aphididae})
PAŠÍKOVSKÝ, Jiří
The aim of this work was a research of the genetic variability of natural populations of Russian wheat aphid Diuraphis noxia (Aphididae) by means of microsatellite markers and markers for EPIC-PCR. First goal was to introduce the methods and optimise them for Diuraphis noxia. In the follow-up pilot study, specimens from 47 lines representing 12 populations from all over the world were analysed. Having used microsatellite markers, I proved expected variability among individual populations and within them. The highest genetic variability was detected between Chile and Algeria using markers for cytochrome C in EPIC-PCR. These findings can be used for further studies of the genetic variability of the aphid Diuraphis noxia.
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Laboratory markers of sepsis
OCHOZKOVÁ, Šárka
Sepsis and its complications are currently the most frequent cause of death owing to multi organ failure at intensive care units. The aim of this thesis was to evaluate the most suitable usable marker with sufficient diagnostic sensitivity and specificity for early catch of sepsis development. For my thesis a chosen data collection patients of intensive care units and patients of neonatology department in Nemocnice a.s. (Hospital Inc.) in České Budějovice was used. In patients, determinations of following concentration parameters were carried out: PCT, CRP, IL-6. For diagnosis and evaluation of clinical state change in relation to sepsis PCT is convenient. For monitoring of steady course and clinical state it is useful and sufficient to watch dynamics of CRP. In newborns the sensitivity PCT decreases in sepsis probably due to immaturity of liver and immunity system. That is why according to experience gained in Nemocnice a.s. (Hospital Inc.) České Budějovice it is more convenient to determine IL-6 instead of PCT.
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The application of AFLP fingerprinting in breeding of Brassica napus
CUŘÍNOVÁ, Petra
AFLP markers are widely used in breeding in some other crops, but their utilization in breeding of Brassica crops is not so frequent. AFLP markers are used for molecular characterization of particular varieties or genotypes and for evaluation of genetic diversity. The aim of this thesis was the application of this method in breeding of rapeseed and in comparative study of genetic variability of different oil seed rape cultivars of Czech, Czechoslovak and German origin. AFLP is based on selective amplification of genomic DNA. Technique consists of three basic steps: 1. restriction of genomic DNA on smaller fragments and ligation of adaptors; 2. selective amplification of fragments with specific primers and 3. electrophoresis of products and computer analysis (Vos et al., 1995). In this thesis 16 Czech (CZ) and Czechoslovak (CZS) and 16 German (G) cultivars of oilseed rape were compared. Two specific primers, EcoRI AGC and fluorescence labeled primer MseI ACC, were chosen for AFLP method. It was possible to distinguish group of CZ/CZS and G cultivars, which have origin in gene resources from Czech Republic and Germany. AFLP technique allows detection of very small differences in Brassica genome. The differences between studied cultivars exist, but they are not very distinct. They show on certain relationship and reduction of genetic basis of genetic resources, which are used in Central Europe.
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