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Performace measurement and assessement of catering services in the two following establishments - Children's Stacionary and The Center of Social Serrices.
HOUDKOVÁ, Ester
In the course of human history man has been changing his choice of food, the way of its acquisition and preparation. At present nutrition is considered to be a part of our lifestyle, on the one side it presents a source of energy, on the other side a source of vital nutrients, vitamins and minerals. Nutrition is widely affected by the socio-economic terms and conditions of the particular individual, at the same time it is a factor with a strong impact on man not only in terms of meeting his basic needs, but also in terms of his psychology. Nowadays, nutrition presents a widely discussed topic with respect to the risks of civilisation disorders, obesity in particular. Current professional estimates suggest that nutrition participates in the health condition of population by 40 to 60 percent. The objective of my work consists in mapping and comparing the conditions of operation in two social care institutions providing catering services, namely ``Dětský stacionář Světluška o.p.s.{\crqq} and Social Care Centre Empatie, which is a part of the social care institution Libnič. The technical and operational conditions in terms of public health protection standards as related to the current legislation were dealt with. In both studied institutions an analysis and evaluation of the all-day catering of clients in compliance with the principles of healthy nutrition including drinking regime were carried out. The intake of milk, milk products, fruits and vegetables, wholemeal products (bakery products in particular), the introduction of fish meals to the menus as compared with the standards of healthy nutrition and the food pyramid were studied. The last but not least criterion of the study was the finding that clients, due to lack of exercise, tend to show a higher body mass index {--} a heavier weight {--} as compared with the recommended values. 80 clients were monitored in the Empatie institution, both children and adults, 30 clients with their age ranging between 4 and 29 years were studied in Světluška. The Hypotheses H2 and H3 predicting that all-day catering menus would show insufficient intake values of milk, milk products, fruits, vegetables, fish and wholemeal products on the one side, and on the other side that the technical and operational conditions of rendered catering services in terms of public health protection standards would comply with the valid legislation could be confirmed by the research carried out in both social institutions. The theory of Hypothesis H1 related to the evaluation of the weight values of clients in both institutions due to lack of exercise has been disproved. The research results will be provided both to the two studied institutions for the purposes of increasing their quality of rendered services, and to the Regional Authority of Public Health and Hygiene KHS of the South Bohemian Region based in České Budějovice. Here they can serve as a source of information as related to the elaboration of check plans. The results can also be used as data for further research and studies in the field of preventive supervision.
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Eating habits of children of II. level of primary schools in Benešov region
PROVAZNÍKOVÁ, Jana
The aim of this thesis is to identity dietary habits of children of second level of primary schools in Benešov region. Firstly theoretical findings from the area of nutrition and dietary habits. The theoretical part is devoted to these findings of the thesis. The next step was the selection of suitable primary schools of Down and village environment, where the surfy could be couducted. Altes meeting the responsible autority, of the schools, letters for parents were given to children, which was Informix then about the thesis topic and was requesting agrément or disagreement of the participation on the surfy. In the case of positive response the children were given a template for filling in the diet and food consumed with user instructions. After the return of these, it was neceséry to process them because of mistakes that appeared and made the data unsuitable for analysis. The diets were evalnated with the aid of the nutrition program found on www.flora.cz. During the evelnation, I have focused especially on the daily energy income and income of individual macronutrients. The results were used in the practical part of my thesis. We will find for example the outcome of individual macronutrients, energy income and the most consumed type of vegetables.
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Eating habits of Primary School Pupils in the Region Písek
MOUDRÁ, Táňa
The main objective of this bachelor thesis was the exploration of the primary school pupils' eating habits. The first task was to review the available sources and definite the basic terms. This task is treated in the theoretical part of the thesis. The following step was the selection of convenient primary schools and the particular research. I also had to write a letter for the children's parents because they had to approve the research. It was necessary to reduce and evaluate the reports. Some of them were useful for the research. The correctly completed forms with three-day records were evaluated into the electronic tables by the nutrition program available at the website www.flora.cz. I was primary interested in the energy intake of he researched group and in its macronutrients intake. The collected data were processed in the practical part of the thesis. The practical part contains for example the comparing of the researched schools. It compares the energy entry in the labour days and at the weekends. There also can be found the summary of the most often consumed drinks and meals. According to my opinion this thesis could be useful in the process of the menus set-up and its modifications.
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Eating habits of Secondary School Pupils in Prague
JÍROVÁ, Lucie
A brief outline of youth nourishment, important nutrients, vitamins and minerale. The text is divided in a way so that the reader can get a clearly organized view on youth nourishment and its recommended amounts. The purpose of this project is to give its readers a current recommendation regarding nourishment, and also to gather all modern scientific information about food and healthy nourishment so that it can be used easily in common life. The main part of the thesis concentrates on a research of the nutrition habits of the youth including its final evaluation. In conclusion the thesis compares pieces of nutrition knowledge with the research results.
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Effect of organic and conventional potato (Solanum tuberosum L.) farming on selected characteristics of potato tuber proteins
MÍČKA, Tomáš
Diploma thesis follows the influence of organic and conventional potato management (Solanum tuberosum L.) on tuber protein content. Potato protein represents from 1/3 to {$^1\!/\!_2$} of total tuber nitrogen compound and belongs among the most valuable plant proteins - its high value is given by high level of essential amino acids. Important group of potato proteins is a patatin (complex of patatin proteins), which is considered as a major storage protein and has many important physiological functions such as antioxidant activity and function of defense system against pathogenic organisms. The aim of the the work was to determined content of crude protein, pure protein and patatin proteins representation in the total protein of potato tubers. The diploma thesis was solved using 5 potatoes varieties, 2 year{\crq}s repetition and variant of organic and conventional potato production system. The obtained data were statistically evaluated. It was found, that content of dry matter in potato tubers was significantly influenced by a year and locality of the field experiment. Crude protein content in potato tubers was significantly influenced by a year, variety and type of potato management system. The potato tubers produced under conventional and organic management contained 9.93 and 9.16 % of crude protein in dry matter, respectively. Pure protein content was significantly influenced by variety. The affect of crop management on the pure protein content was not statistically signifiant. However, slightly higher content of pure protein was evaluated in dry matter of potato tuber produced under convetional than under organic crop management resulting in average value of 4.23 and 3.86 %, respectively. Representation of pure protein in total content of nitrogen matters was significantly influenced by variety and a year of growing. Complex of patatin proteins (40-45 kDa) was detected for all of the evaluated potato varieties and variants of the field experiment. Higher number of patatin isoforms (with rather physiological function) was investigated in potato tubers from organic crop management.
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Pregnant and nursing woman´s general model of diet.
VRÁBKOVÁ, Petra
The principal aim of this bachelor thesis was at first analysis of literature concerning the subject of pregnant and nursing woman. The opinion poll with 27 questions followed after that. It should validate or overcome three theories. The first one was related to the presumption, that the supplements of food and the better way of feeding during the pregnancy influence the tiredness of the pregnant woman and also the proper process of the pregnancy. This theory stayed unconfirmed. The second hypothesis is looking for an answer to the question, if the length of breast feeding was influenced by the nursing mother´s right way of living. The second theory was not confirmed by the partial questions. The third hypothesis was dealing with food supplements used during breast feeding and their influence on the quality of hair, nails, teeth and skin. In this case there was found some context.
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Characterization of 18,7 and 19 kDa groups of secreted proteins in the salivary glands of the castor bean tick \kur{Ixodes ricinus}
SOUČKOVÁ, Nina
The recombinant protein c90 was prepared and polyclonal antibodies against this protein were raised. The dsRNA was made for the experiments with RNA interference. The samples from dissected tissues of dsRNA silenced ticks were tested by RT-PCR and Western blot. Results suggest that protein c90 plays a role in the tick body during the reaction to injury. Finally, another experiment with injection of water, G+ and G- bacteria into the ticks was realized. It was found that the members of the 18,7 kDa protein family can create multimers. The overexpression of silenced genes was observed during RNAi experiments despite of expected inhibition of c90 production. These results together with the bioinformatics analysis could mean that these proteins are important the physiology of tick probably as a reaction to injury. However c90 protein is produced only in the first phase of feeding which could mean that it has some role in the tick-host interaction as well.
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Cold hardiness of larvae of the fruit fly, \kur{Drosophila melanogaster} (Diptera: Drosophilidae)
KORBELOVÁ, Jaroslava
We assessed survival of larvae of the fruit fly, Drosophila melanogaster at low temperatures (0°C and 5°C) after rearing them under seven different acclimation regimes. Larvae that have developed in a standard diet at 25°C showed 50% mortality after 12,6 min of the exposure to 0°C (Lt50 = 0.21 h). In contrast, larvae that have developed in a diet enriched with glycerol at 15°C, and were cold acclimated at 5°C during last two days of their development, had Lt50 = 38.6 h. It means that it was possible to increase the Lt50 at 0°C more than 180-fold using simple manipulations with rearing temperatures and diet composition. The physiological differences in duration of larval development, fresh mass, dry mass, hydration and total contents of proteins, lipids and glycogen between the larvae belonging to different acclimation variants are described. The samples for future detailed metabolomic analysis were prepared.
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