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Utilization of confocal microscopy to study of degradation of organic dye in cell biology
Trnová, Kateřina ; Provazník, Ivo (referee) ; Čmiel, Vratislav (advisor)
This thesis focuses on the study of the life of a fluorescent dye. It also examines changes in its emission spectrum determined with the aid of development of fluorescence intensity over the long term sensing in several kinds if of cella. The general part of the thesis with the issues of confocal microscopy, fluorescent radiation and maks. Furthermorer, an analysis of methods for measuring fluorescence lifetime is done. In the thesis there is elaborated a description of the program, whitch was designed for the analysis of the collected output of the used confocal microscope. Sebsequently, the results are evaluated.
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Landscape of mining
Frič, Miloslav ; Ryška, Pavel (referee) ; Vaněk, Vojtěch (advisor)
The landscape of surface mining is a visual play that expoits the land on this large format drawing. My work investigates the limits of hanged art piece through the use of video projection and camera. The spectator is not limited by a clear thought framework but urged to experience a visual experience in this very place and time.
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Renewal of hermetic pipe penetrations
Kratochvíl, Zdeněk ; Chladil, Josef (referee) ; Kalivoda, Milan (advisor)
The topic of this work is the renewal of hermetic pipe penetrations within the primary loop of a nuclear power station. A description of the Dukovany nuclear power plant is included at the beginning, ranging from basic description to details of the renewed component and the reasoning for its replacement. Following is a description of the technologies, which are applied in order to restore the hermetic pipes, the choice of the placement of the heterogeneous weld and the compilation of a workflow for the renewal in the environment of the primary loop while maintaining the quality standards and the strict safety conditions. The conclusion includes a calculation of the expenses tied with the chosen variant the reasoning behind the renewal regarding the length of the outage.
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Optimization of core molds for injection molding
Stavárek, Václav ; Vaculka, Miroslav (referee) ; Vosynek, Petr (advisor)
Diplomová práce vznikla ve spolupráci s průmyslovým partnerem, který vyrábí elektrické komponenty pro automobilový průmysl. Tato firma se potýká s problémy často se porušujících jader v některých jejich formách na vstřikování plastů, vyrábějících převážně housingy pro konektory. Firma disponuje licencemi na komerční software pro simulaci injekčního vstřikování Moldflow a Moldex3D a také pro simulaci metodou konečných prvků Ansys. Nejprve jsou shrnuty teoretické poznatky ohledně injekčního vstřikování a jeho simulace, řešení problémů interakce těles s tekutinou a únavy materiálu. Poté je popsán proces stanovení únavové životnosti jádra formy s využitím výše zmíněného softwaru. Proces je vysvětlen na příkladu konkrétní formy ve výrobě této firmy. Je zvolen takový přístup vyhodnocení únavy, který nejvíce odpovídá současné životnosti jader, a ten je pak použit pro analýzu vlivu změny geometrie jádra a parametrů vstřikování. Změny ostatních parametrů, které simulace neumožňuje zahrnout, jsou rovněž navrhnuty a pokud možno odůvodněny jinými způsoby. Jedno z doporučení je přidání zaoblení na obě jádra, což by mohlo prodloužit životnost toho problematičtějšího z nich z 30 dnů na více než 320 dnů. Toto by mohlo znamenat úspory až 10 600 EUR ročně. Další doporučení je změnit způsob obrábění jader a také přidat jejich tepelné zpracování.
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Procedure of residual fatigue lifetime assesment of railway axle
Scholz, David ; Seitl, Stanislav (referee) ; Náhlík, Luboš (advisor)
The goal of the bachelor's thesis is to present options to calculate residual life of a railway axle from more sophisticated points of view. These methods are requested by railway operators to ensure higher safety of train traffic. The main issue is the perspective of fracture mechanic, considering railway axle as a component with an initial crack or other defect. The theoretical part deals with basic definitions of fracture mechanics and processes describing crack propagation, using conception of stress intensity factor, which is the most used nowadays. Following part also describes a fatique life estimation process in real. This process is demon-strated using an example after that.
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Stability of vesicular systems using fluorescence spectroscopy techniques
Máčala, Jakub ; Venerová, Tereza (referee) ; Mravec, Filip (advisor)
This thesis is focused on possibility of studying stability and fusion of catanionic vesicles with Förster resonance energy transfer. The mainly used technique was Time-Correlated single photon counting. Firstly, excitation and emission spectra of chosen probes were measured and donor-acceptor pairs were suggested: 5-hexadecanoylaminofluorescein with Octadecyl Rhodamine B, Bodipy 493/503 with rhodamine or DiI, perylene with fluorescein, DiO with DiI. Then, time-resolved measurements of suggested pairs from environment of catanionic vesicles with different content of cholesterol were made in order to track the FRET associated with fusion of vesicles. It was found out, that it is not possible to use DiO as a donor because of it’s inefficient solubilisation into vesicles. It is also not possible for Bodipy to be used as a donor, because of it‘s excimer formation. In case of using fluorescein as a donor, it was found, that there is ongoing homo-fret between fluorescein molecules. Thanks to this, fusion was tracked by addition of unstained vesicles. It was also possible to track fusion in longer period of time. Also perylene-fluorescein pair was found to be capable of tracking the fusion, but with the exception of vesicles with content of cholesterol of 43 mol. %, tracking of fusion was possible only in short period of time.
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Optimalization of power supply for LED light sources
Koždoň, Jakub ; Kosina, Petr (referee) ; Boušek, Jaroslav (advisor)
This work focuses on description of function, analysis of degradation process, measuring lifetime and design thermal and electrical driving circuit for LED. Lifetime power LED basically depends on their working temperature. So some methods for measuring and determine LED lifespan and impacts which affects their lifetime are going to be described. Driving circuits are one of keys of element lamps. Their characteristics can affect lifetime of all lamp. So some properties and impacts of circuits for driving LED's and their functions which are necessary need to know in design will be described . I'll also catalogue some circuits which are suitable for that and show particular design power supply for LEDs and it's realization.
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A light source with variable intensity
Koždoň, Jakub ; Mojrová, Barbora (referee) ; Boušek, Jaroslav (advisor)
This work is focused on description how the light impact human live, description of light sources and finally design and construction of light source with adjustable colour temperature and luminous flux. In introduction is mentioned the process of vision and how the light impact humans. In next part is characterization of currently used light sources, their construction, properties, benefits and drawback with their main parameters. Important part of this chapter is more extensive description of light emitting diodes with their operational parameters. The last two parts is focused on design and measuring the parameters of created light source with adjustable colour temperature and luminous flux and evaluating the results.
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