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Feasibility of performing laboratory tests in the paediatrician´s office
HOLOUBKOVÁ, Hana
The thesis deals with feasibility of performing laboratory tests in the paediatrician´s office. The objective of my thesis was to learn about the current state of feasibility of performing laboratory tests right in the paediatrician´s office. To meet this objective I distributed questionnaires and visited twenty randomly selected pediatricians. Thereby I got familiar with the structure of tests used in paediatric practice. I have learnt to perform and interpret relevant laboratory methods. The theoretical part of the thesis gives an account of examinations carried out in paediatricians´ offices. It deals in detail with laboratory analyses performed on urine, blood, stool and swab samples. The practical part gives a description of techniques of particular methods and their current use in paediatricians´ offices. These methods are chemical examination of urine by using urinalysis reagent strips, examination of the urine sediment, Uricult examination, C-reactive protein determination, determination of sedimentation rate, determination of glycemia using glucometer, test for occult bleeding, rotavirus and adenovirus assay, Dentocult examination and detection of Streptococcus pyogenes. The results shown in graphs illustrate how frequently the methods are performed.
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Laboratory markers of sepsis
OCHOZKOVÁ, Šárka
Sepsis and its complications are currently the most frequent cause of death owing to multi organ failure at intensive care units. The aim of this thesis was to evaluate the most suitable usable marker with sufficient diagnostic sensitivity and specificity for early catch of sepsis development. For my thesis a chosen data collection patients of intensive care units and patients of neonatology department in Nemocnice a.s. (Hospital Inc.) in České Budějovice was used. In patients, determinations of following concentration parameters were carried out: PCT, CRP, IL-6. For diagnosis and evaluation of clinical state change in relation to sepsis PCT is convenient. For monitoring of steady course and clinical state it is useful and sufficient to watch dynamics of CRP. In newborns the sensitivity PCT decreases in sepsis probably due to immaturity of liver and immunity system. That is why according to experience gained in Nemocnice a.s. (Hospital Inc.) České Budějovice it is more convenient to determine IL-6 instead of PCT.
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