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The serotonin transporter gene - polymorphism incidence study.
Bílá, Nikola ; Macek, Milan (advisor) ; Goetz, Petr (referee)
Serotonin (5-hydroxytryptamine; 5-HT) is a neurotransmitter in the central and peripheral nervous systems. Serotonin transporter 5-HTT plays an important role in serotogenic transmisssion and the serotonin transporter gene is under investigation in connection with multiple psychiatric disorders. Three polymorphisms, an insertion/deletion and SNP in the promoter region and a variable nucleotide tandem repeat (VNTR) in intron 2, influence expression of the 5-HTT gene. We examined these polymorphisms at the serotonin transporter protein locus (SLC6A4) in three specific and representative cohorts drawn from the Czech population. These cohorts were stratified according to their age: a) randomly selected elderly individuals (over 75 years of age), b) young adults (age ranging between 19- 45 years) and c) newborns, all with balanced gender representation. The two regions were amplified and resolved on a 2% agarose gel and/or analyzed using the capillary electrophoresis in order to discriminate lenght polymorphisms. SNP in intron 2 was examined by RFLP after using MspI restriction. This analysis revealed that elderly individuals were statistically more likely than controls to carry two copies of the L allele of the lenth polymorphism. This may be due to the protective character of L allele, which is associated with...
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The serotonin transporter gene - polymorphism incidence study.
Bílá, Nikola ; Macek, Milan (advisor) ; Goetz, Petr (referee)
Serotonin (5-hydroxytryptamine; 5-HT) is a neurotransmitter in the central and peripheral nervous systems. Serotonin transporter 5-HTT plays an important role in serotogenic transmisssion and the serotonin transporter gene is under investigation in connection with multiple psychiatric disorders. Three polymorphisms, an insertion/deletion and SNP in the promoter region and a variable nucleotide tandem repeat (VNTR) in intron 2, influence expression of the 5-HTT gene. We examined these polymorphisms at the serotonin transporter protein locus (SLC6A4) in three specific and representative cohorts drawn from the Czech population. These cohorts were stratified according to their age: a) randomly selected elderly individuals (over 75 years of age), b) young adults (age ranging between 19- 45 years) and c) newborns, all with balanced gender representation. The two regions were amplified and resolved on a 2% agarose gel and/or analyzed using the capillary electrophoresis in order to discriminate lenght polymorphisms. SNP in intron 2 was examined by RFLP after using MspI restriction. This analysis revealed that elderly individuals were statistically more likely than controls to carry two copies of the L allele of the lenth polymorphism. This may be due to the protective character of L allele, which is associated with...
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Psychological specifics of teacher educating children with autistic disorders at a special scholl
Bílá, Nikola ; Šírová, Eva (advisor) ; Mertin, Václav (referee)
The theoretical part of the thesis concentrates at the begining on pedagogical profession in general with deeper focus on a repertoire of techers' activities. There are being charecterised mandatory personal presumptions and competences of teachers. In detail is elaborated the part about autism. The main part of the chapter constitutes of a review of psychological and medicinal aproach to this issue, diagnostical criteria and a overview of all the pervasive developmental disorders. The final part describes the possibilities of educating children with autism not just in the Czech republic but as well in other european countries. The empirical part of the thesis shows results of qualitative research focused on evaluation of psychological specifics of the work of teachers educating children with autism in the Czech republic from the point of view of the teachers and parents. The data were collected by observation, interview and analysis of documents. KEY WORDS Teacher, working competences, autism, educating children with autism.
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