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SPE Utilization in Analysis of Benzimidazoles I
Janoušek, Jiří ; Kubíček, Vladimír (advisor) ; Drastík, Martin (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Candidate: Jiří Janoušek Supervisor: Ing. Vladimír Kubíček, CSc. Title of diploma thesis: Use of SPE in analysis of benzimidazoles I Anthelmintic resistance in parasitic worms is an up-to-date topic. In order to combat this resistance we need to find proper analytic methods of its monitoring. The goal of this thesis is to find a suitable method of benzimidazole extraction, using a solid-phase extraction. During the experiments I used SPE tubes Supelco DSC-C8 (1ml, 100mg). In my thesis I tested the influence of pH and composition of elution agents on recovery of extraction. I performed the extraction in acid, neutral and basic environment. Elution agents consisted of a mixture of acetonitrile and polar phase, with four different mixing ratios. The best results were obtained during extraction in basic environment. For SPE tubes DSC-C8 the most suitable elution agent consisted of 4 parts of acetonitrile and 6 parts of 10% ammonia solution. Keywords: benzimidazoles, flubendazole, mebendazole, solid-phase extraction, resistance, anthelmintics
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Study of the effect of the amount of chelating agent addition on the result of monoclonal antibody conjugation
Janečková, Adriana ; Bárta, Pavel (advisor) ; Janoušek, Jiří (referee)
v anglickém jazyce Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Mgr. Adriana Janečková Supervisor: Mgr. Pavel Bárta, Ph.D. Title of rigorosum thesis: Study of the effect of the amount of chelating agent addition on the result of monoclonal antibody conjugation Monoclonal antibodies have become the important tool not only for the therapy, but also for the diagnosis of the infectious, autoimmune or cancer diseases in the last few decades. In addition to their own therapeutic effect, monoclonal antibodies are used as vectors for biological substances with therapeutical and diagnostic importance. Besides bacterial and plant toxins, monoclonal antibodies are also effective in their capability to deliver radionuclides to the site of their specific binding, i.e. the antigen against which they were prepared. The so called radioimmunoconjugates are then successfully used in radiodiagnostic and radiotherapeutical field. Radionuclides can be bound to antibody molecule either directly in the case of halogenation or indirectly. The latter method is the most common one due to a greater stability and suitability for more radionuclides, which can be transported by antibodies. Substances that allow the radionuclide biding to the monoclonal antibody...
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Phenolic acids in plants
Klížová, Lucie ; Karlíčková, Jana (advisor) ; Janoušek, Jiří (referee)
CHARLES UNIVERSITY PHARMACEUTICAL FACULTY IN HRADEC KRÁLOVÉ DEPARTMENT OF PHARMACEUTICAL BOTANY Title of the Diploma thesis: FENOLIC ACIDS IN PLANTS Candidate: Lucie Klížová Supervisor: PharmDr. Jana Karlíčková, Ph.D. Diploma thesis 2020/2021, pp. 153 Phenolic acids belongs between secondary metabolites, which are part of fruits, vegetables, cereals and other plants of different families. For example, it can be plants of family Lamiaceae, Asteraceae and Ericaceae, which contain significant amounts of these substances. In plants phenolic acids play an important role and they have many human health-promoting effects including antioxidant, anti-inflammatory, antiviral, antibacterial, antifungal, anticancer activity and other. Plants containing phenolic acids are preferably also used as antidiabetics, expectorants, insecticides, hypolipidemics, cardioprotective and neurological compounds, etc. The content of these compounds in plants may be different according to monitored cultivar, the place of growth, the time of harvest and many other aspects. Presence, identification and subsequent quantification of individual phenolic acids are usually done through the HPLC and MS methods and total content of these compounds is determined through the Folin-Ciocalteu method. The diploma thesis is a literature...
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Drone swarm control
Kolísek, Josef ; Marcoň, Petr (referee) ; Janoušek, Jiří (advisor)
This thesis focuses on the drone swarm control and the using in practice. It is about the interconnection of several control units of drones and subsequent coordination in the air. The work is divided into theoretical part and practical part. The practical part describes the possibilities of drone swarm flying and coordination of multiple drones. Possible ways of communication and transmission of telemetry data for control using the MAVLink protocol. The next part describes the possibilities of controlling multiple units from one central point and creating flight instructions. The practical part deals with the realization of the software for synchronized flight of two unmanned aircraft and their connection with the ground control station for the transmission of flight instructions. The last part of the work deals with evaluation of the control, compliance of the established flight plan and the proposal of other possibilities for improvement with the solution of deficiencies.
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Vision-Based Autonomous Navigation Of An Unmanned Aerial Vehicle
Janoušek, Jiří
This article is focused on the real-time detection and recognition of the objects by using acamera located on Unmanned Aerial Vehicle (UAV). The detected objects in the image carry informationabout the next points of the flight plan according to which the drone performs an autonomousflight. The drone can recognize the information encoded in the patterns on the ground by the field ofview of the camera, therefore, it can perform flight manoeuvres consequently. The detection andinformation recognition is performed by a single-board computer and it is based on comparing imageswith a predefined Haar cascade. The single-board computer on the drone recognizes a size andspacing of the individual contrast points which determine the partial information about the next flightpath. Afterward, single-board computer transmits the detected information to the Pixhawk controlunit by using the Mavlink protocol in real-time. The aircraft control unit ensures the accurate positioningof the UAV by sequential execution of the commands.
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Production of pressure filter casing
Janoušek, Jiří ; Forejt, Milan (referee) ; Podaný, Kamil (advisor)
The project presents a proposal for the technology of production of the pressure filter casing used in hydraulic systems, which is made of non-alloy carbon steel 12 020. With the help of a literature study of cold extrusion and calculations, the production of the component was designed, which will take place in two operations. Before each operation, the surface treatment is performed by phosphating and soaping and inter-operational annealing must take place. Tool sets were designed for both operations and a press was selected from the company's machine park with a nominal force of 25,000 kN. Finally, a technical and economic evaluation was proved, which shows that the price of production of one piece is CZK 187. The turning point was set at 3 497 pieces per year.
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Drone swarm control
Kolísek, Josef ; Raichl, Petr (referee) ; Janoušek, Jiří (advisor)
This thesis focuses on the drone swarm control and the using in practice. It is about the interconnection of several control units of drones and subsequent coordination in the air. The work is divided into theoretical part and practical part. The practical part describes the possibilities of drone swarm flying and coordination of multiple drones. Possible ways of communication and transmission of telemetry data for control using the MAVLink protocol. The next part describes the possibilities of controlling multiple units from one central point and creating flight instructions. The practical part deals with the realization of the software for synchronized flight of two unmanned aircraft and their connection with the ground control station for the transmission of flight instructions. The last part of the work deals with evaluation of the control, compliance of the established flight plan and the proposal of other possibilities for improvement with the solution of deficiencies.
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Object recognition based autonomous control of an unmanned aerial vehicle
Klouda, Jan ; Marcoň, Petr (referee) ; Janoušek, Jiří (advisor)
This thesis deals with the control of an autonomous drone based on image recognition. The goal is to create a program in the Python programming language that determines the position of an object in the field of view of the camera. The autonomous drone then performs a predefined operation. The work is divided into theoretical and practical part. The theoretical part describes the methods of image processing and evaluation of objects, the resulting methods of communication between the control station and the drone. The practical part describes the implementation of autonomous control of the aircraft based on the detection of objects in the image. The last part deals with the evaluation of successful detection objects and the control accuracy of the created solution.
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Factual findings and legal assessment
Janoušek, Jiří ; Winterová, Alena (advisor) ; Dvořák, Bohumil (referee)
FACT-FINDING AND LEGAL ASSESSMENT Abstract According to the difficulty of the subject and key question being discussed in this study, it is not the aim of this work to come up with one perfect solution of distinguishing between factual and legal question during an application of law in civil procedure. The theme of this study implies that the main goal is to get as close to actual explanation of any potential solution as possible, using predominantly an analytical method of legal research, and to approach this subject also from less traditional points of view. This work is focused on one of fundamental problems of procedural law which also has deeper theoretical basics. It starts with a typical scheme of an application of law where the factual question (consisted of perceiving and evidence) and legal question (where the iura novit curia principle is applied). After finishing the fact-finding process, these findings are subsumpted under a general legal norm. A subsumption itself is the centre of the problematics of incorrect legal assessment. The study also includes a different view on this classical model of application of law. A precise distinguishing between factual and legal questions in civil procedure has a big influence on several areas of civil procedure, while the most important part is the only...
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Autonomous Charging Stations For Drones
Janoušek, Jiří
This article deals with autonomous charging station for drones. In the first one, the term "charging station" is analyzed in the context of its applicability. Then, section characterizes the process of charging and description of each parts of the charging station. The final part of the thesis presents appropriate method for precision landing on the charging station. The thesis presents the designing and fabrication of an automatic charging station for the unmanned aerial vehicles.
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