|
| |
|
|
The use of CAM assay for characterization and study of cancer cell invasive properties
Vágnerová, Lenka ; Dvořák, Michal (advisor) ; Geryk, Josef (referee)
The chorioallantoic membrane (CAM) of chicken embryos belongs to the in vivo model systems frequently used for the study of angiogenesis and cell invasiveness. Using CAM assay we have tested selected chicken sarcoma cell lines characterized by different angiogenic properties and different ability to form metastasis. In addition to CAM assay, several other methods have been used to characterize the phenotype of these cell lines. We have selected a few proteins which could significantly influence the angiogenic and metastatic properties of investigated cell lines. We have established cell lines stably overexpressing these genes and compared their phenotypes with parental cell lines. We have shown that genes encoding ISL1, ARNT2, PROM1, HOXA11 proteins participate, in our experimental model, in activation of programes controlling angiogenesis and cell invasion.
|
|
|
Gene expression in chicken embryo: micromanipulation and visualization methods
Bendová, Michaela ; Dvořák, Michal (advisor) ; Hirsch, Ivan (referee) ; Krylov, Vladimír (referee)
The aim of this work was to obtain better insight into the principles of cell structures and organs in the chicken embryo development. To reach this goal special methods of micromanipulations and visualization in vitro, ex vivo, ex ovo and in ovo were implemented and adjusted. These methods were used to study gene expression in neural crest development and eye development. In the course of long term research in our laboratory we observed that oncoprotein v-Myb influences the development of the neural crest and has the capacity to change natural cell fate. We performed a series of experiments to investigate v-Myb protein influence on neural crest cells differentiation, especially melanocyte lineage development, and its influence on gene expression in the neural crest. Therefore we focused on Gremlin 2 (PRDC), the gene upregulated by v-Myb in the neural crest. The established procedure of electroporation in ovo was adjusted to transfect cells of the developing eye and used to study gene expression during lens induction. The results obtained from chicken embryo experiments endorsed the study performed on mouse embryos. Futhermore, the electroporation technique was slightly modified for manipulations of the neural retina in the developing eye in ovo. Thereafter, the retinas were processed ex vivo and...
|
|
|
Cytokine expression in chemically-induced senescence
Nováková, Zora ; Hodný, Zdeněk (advisor) ; Hampl, Aleš (referee) ; Dvořák, Michal (referee)
AbstractJanderova-Rossmeislova L., Novakova Z., Vlasakova J., Philimonenko V., Hozak P., Hodny Z., 2007. PML protein association with specific nucleolar structures differs in normal, tumor and senescent human cells. J. Struct. Biol. 159, 56-70. Cellular senescence, widely recognized as a potent suppressor of tumorigenesis, represents response to cellular stress and DNA damage which results in irreversible cell growth arrest. Growing evidence signalizes crucial role of cytokine production in senescence phenomenon. Recently, many research groups have efforted to define exact character of senescence-associated secretory phenotype and its function in senescence development and maintentance. Factors secreted by senescent cells, mainly of proiflammatory character, were found to have pronounced effects on their environment as well as on its own producer. These observations were obtained preferentially on models of replicative senescence and oncogene-induced senescence. Vlasakova J, Novakova Z, Rossmeislova L, Kahle M, Hozak P, Hodny Z. 2007. Histone deacetylase inhibitors suppress IFNalpha-induced up-regulation of promyelocytic leukemia protein. Blood 109: 1373-80 Novakova Z, Man P, Novak P, Hozak P, Hodny Z. 2006. Separation of nuclear protein complexes by blue native polyacrylamide gel electrophoresis....
|
|
|
Study of extracellular nucleic acids in maternal circulation in the cases of pathological and physiological pregnancies
Žejšková, Lenka ; Hromadníková, Ilona (advisor) ; Černá, Marie (referee) ; Dvořák, Michal (referee)
In 1997, Prof. Dennis Lo discovered the presence of cell-free fetal DNA (cffDNA) in the maternal plasma and serum of pregnant women. This finding started the development of new non-invasive prenatal diagnosis methods, which are currently in the forefront of the advanced care of mother and fetus. Non-invasive genetic tests based on the detection of paternally inherited alleles, including determination of fetal sex in cases at risk of X-linked disorders or congenital adrenal hyperplasia and RHD or RHCE genotyping in alloimunized pregnancies, were quickly introduced into routine practice. This thesis focuses on the basic characteristics of cffDNA and fetal cells in maternal circulation and its usage for non-invasive prenatal diagnosis, especially in cases of placental insufficiency related complications, e.g. preeclampsia and IUGR. This severe disorder is characterized by placental dysfunction with an abnormal invasion of trophoblasts and a defect in the transformation of maternal spiral arteries, leading to placental ischemia followed by increased apoptosis of trophoblast associated with an elevated concentration of cell-free nucleic acids in maternal circulation. Until recently, cffDNA quantification studies were mostly done using amplification of SRY or DYS-14 genes localized on chromosome Y, and...
|
|
| |
|
|
Design of home astronomical clock
Dvořák, Michal ; Řezníček, Svatopluk (referee) ; Rajlich, Jan (advisor)
The topic of this bachelor thesis is a design of a multifunctional table astronomical clock. The main goal is to design an object displaying astronomical data in a modern design, which does not currently exist on the market. The work is inspired by historical astronomical clocks in the form of tower clocks and at the same time uses the possibilities of modern technologies. The resulting product will serve as a complement to social or domestic environments.
|
|
|
Interactive flexible program - Sulfur chemistry and its impact to acquiring of curriculum
Dvořák, Michal
UNIVERZITA cká fakulta KARLOVA V PRAZE, Přírodověde Katedra učitelství a didaktiky chemie CHA ce RLES UNIVERSITY IN PRAGUE, Faculty of Scien Department of Teaching and Didactic of Chemistry Interaktivní flexibilní program - Chemie síry a jeho vliv na efektivitu osvojování učiva Interactive flexible program - Sulfur chemistry and its impact to acquiring of curriculum Mgr. Michal Dvořák Souhrn disertační práce Summary of PhD. Thesis 1 Praha/Praque 2009 2 KEYWORDS Interactive flexible program; electronic materials; digital textbook; education software; effectiveness; process of acquiring curriculum; key competencies; chemistry xperiments, personal computer; technology. e INTRODUCTION We live in an era of information technology where the revolutionary way of acquiring new information is available. The new information deepen and widen acknowledge in individual branches of science. Most of the new information contradicts the validity of the existing data. Huge increase and still rising difficulty of the new information require the fundamental changes in the organization of process of acquiring curriculum. But the existing education tools specified for self-studying pupils do not reflect these...
|
|
|
Manipulator Control for Acquisition of the Eye Retina
Malaník, Petr ; Dvořák, Michal (referee) ; Drahanský, Martin (advisor)
To use the image of the retina for biometric or medical purposes, it is necessary that the image is of the highest quality and ideally covers the largest possible area. It is therefore necessary to move the sensing device to the most suitable position while ensuring its stability. A laboratory handling platform is used for this purpose. The platform is moved by stepper motors. A control board equipped with a microcontroller has been developed for their control, which allows very fine movements. The theoretical accuracy of the manipulator is up to 20 nm. Since it is necessary for sensing, to have proper retina illumination, the control system also includes adjustable illumination elements in the infrared and visible spectrum. The resulting system allows scanning of the retina of the eye from multiple angles and thereby effectively increase the area on which it is possible to further look for diseases or biometric features.
|
|
|
Firearm Type Identification in an Image
Čech, Ondřej ; Drahanský, Martin (referee) ; Dvořák, Michal (advisor)
Main goal of this work is to design, implement and test an approach for classifying firearms in an image into categories with short and long fireams, and then with single shot, multi-barreled, repeating and semi-automatic/automatic firearms. This problem was solved using SVM classifier together with Harris corner detector, FREAK descriptor and Bag of Words method. Accuracy of final program is up to 13,3 %.
|
guest ::
