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Preparation of nanostructures using nanoporous template
Dzuro, Matej ; Hrdý, Radim (referee) ; Drbohlavová, Jana (advisor)
The aim of the work described in this paper is to prepare electrochemically an optical sensor consisting of TiO2 quantum dots (QDs) using nanoporous Al2O3 template in the first step and to modify the surface of QDs with gold layer by evaporation process, again using nanoporous template, in the second step. This easy, cheap and rapid approach provides homogenous and highly ordered distribution of both nanostructures on substrate. The final objective is to characterize the physical parameters and fluorescent properties of TiO2/Au sensor designed for optical detection of biomolecules such as DNA and proteins.
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Nanoelectrodes for biophysical measurements
Márik, Marian ; Hrdý, Radim (referee) ; Hubálek, Jaromír (advisor)
The aim of the project is formation of nanoelectrodes on the silicon wafer surface by thin-film techniques combined with lithography and nanotechniques developed in LabSensNano. Project is engaged in lithography and in preparation of silicon wafers. Furthermore is proposed a brief proposal of electrodes, proposal of realization and current state of the experiment.
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Preparation Techniques and Characterization of Electrodes with Nanostructured Surface
Hrdý, Radim ; Trnková, Libuše (referee) ; Janderka,, Pavel (referee) ; Hubálek, Jaromír (advisor)
Nowadays, nanostructures fixed on solid substrates and colloidal nanoparticles permeate through all areas of human life, in area of sensors and detection as well. This dissertation thesis deals with the fabrication of nanostructures on the surface of planar electrodes via self-ordered nanoporous template of aluminum trioxide. The nanofabrication, as one of many possible techniques, is used to increase the active surface area of electrodes by creating unique surface types with specific properties. These electrodes are very perspective in the applications, such as biomolecules electrochemical detection and measurement. The transformation of aluminum layer into non-conductive nanoporous template in the process of anodic oxidation is a fundamental technique employed to obtain the array of nanostructures in this thesis. The fabrication of high quality nanoporous membranes with narrow pore size distribution on various types of metallic multilayers is one of the key experimental parts in this work. Several problems associated with the production of the thin-film systems, including the dissolving the barrier oxide layer, are discussed and solved. Another part of this work deals with the use of nanoporous membrane as a template for the production of metallic nanostructures via electrochemical metal ions deposition directly into the pores. The obtained nanostructures as nanowires, nanorods or nanodots are characterized by the scanning electron microscopy and energy-dispersive or wavelength X-ray spectroscopy. The electrode surface, modified by gold nanostructures suitable for the detection of biomolecules, has been chosen for the electrochemical measurements, due to the gold biocompatibility. The nanostructured electrodes were characterized by electrochemical impedance spectroscopy and cyclic voltammetry. The effect of nanostructured surface geometrical parameters, including the size of the electrochemically active area, on the results of electrochemical measurements has been observed and compared to flat gold electrodes. Two model biomolecules, namely guanine and glutathione, have been chosen for the study of potential application of these nanostructures in biosensors.
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Electrochemical impedance spectroscopy as a nanostructured bioelectrodes characterization method
Vrbová, Eva ; Urbánková, Kateřina (referee) ; Hrdý, Radim (advisor)
Diploma thesis deals of nanostructured surfaces, nanoparticles and electrochemical characterization methods such as cyclic voltammetry, differential pulse voltammetry and electrochemical impedance spectroscopy. The aim of this thesis is a theoretical research issues of production and characterization nanostructured modified electrodes. The practical part is the production of biomodified nanostructured electrodes by anodi- zation W/Al layers with galvanic deposition of gold or deposition of mercury, a modifi- cation of the electrodes by 11-mercaptoundecanoic acid and by bovine serum albumin (BSA). The thesis includes SEM images of nanostructured electrodes contact angle mea- surements of these electrodes and form an electrical circuit with subsequent simulation waveforms.
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Nanostructured surfaces for electrochemical detection
Dzuro, Matej ; Hrdý, Radim (referee) ; Drbohlavová, Jana (advisor)
This work deals with the preparation of gold nanostructures for future usage in electrochemical sensors and biosensors, methods for their characterization and production. The emphasis is focused on the template-based electrodeposition method of gold and on study of the effect of manufacturing conditions on physical properties, mainly electrical and topological of nanostructures. Thesis is focused also on overall function and sensitivity of the gold nanostructured electrode.
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Electrochemical Characterization of Nanostructured Surfaces Modified by Substancies with Thiol Bound
Urbánková, Kateřina ; Kynclová, Hana (referee) ; Hrdý, Radim (advisor)
This master thesis deals with nanotechnology, nanoparticles and nanostructured surfaces, electrochemical methods, especially voltammetry, cyclic voltammetry, electrochemical impedance spectroscopy and contact angle measurement. One part is focused on electrodes primarily nanostructured and modified by substancies with thiol bound. Tutorial for preparation of gold nanostructured electrods is introduced in practical section including SEM photos of electrode surface. Nanostructured and bare gold electrodes were modified by 11-mercaptoundecanoic acid, streptavidin, glycine and biotin and measured by cyclic voltammetry, electrochemical impedance spectroscopy and contact angle.
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The electrochemical sensors with 3-D structure of electrodes
Štekovič, Michal ; Hrdý, Radim (referee) ; Adámek, Martin (advisor)
Miniature electrochemical sensors can be produced by thick-film technology. The optimal properties of thick-film electrode system, the production technology optimization of thick-film sensors and the adjustment of optimal technological properties are main problems of sensor design. An area of working electrode and an electrode topology design is the important parts in design of electrode system optimization. The rules for optimization of electrode systems in classical electrochemistry exist. The design of standard thick-film electrode construction is limited (2-D construction, substrate dimensions, resolution, paste parameters …). Therefore rules for classical electrode systems cannot be used fully in this case and larger electrode area can be making by 3D structure only. One of resolutions is unconventional method. The increase of working electrode surface by unconventional method – ball and wire bonding is discussed in this work.
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Impact of input parameters variation on fabrication of nanoporous alumina
Hriczo, Filip ; Macháčková, Marina (referee) ; Hrdý, Radim (advisor)
This thesis examines and tests acids and conditions, which make the production of nanoporous ceramic at the thin aluminium layer the most effective and provide the highest quality. This paper describes the production of nanoporous structures with pore size 15-400 nm, depending on the electrolyte. Creating a regular hexagonal structure by electrochemical oxidation is dependent on many parameters that affect the regularity of structure and parameters of the ceramics produced. They were investigated primarily by changes in temperature and input voltage. All results were examined by SEM analysis.
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Nanotechnology in construction of sensors for detection of hydrogen
Macháčková, Marina ; Hrdý, Radim (referee) ; Hubálek, Jaromír (advisor)
Aligned arrays of nanostructures has recently attracted great interest because of their unique properties and potential use in a broad range of technological applications. The nanostructures can be employed when it is essential to create large surface on a small area in electronic device as sensor technology or energetics e.g. solar panels. One of the simplest and low-cost methods of fabricating nanostructures is template-assisted electrochemical deposition. This method also enables good control over the nanostructure dimensions and can be used to deposit a wide range of materials. The proposed method consists of two steps. At first, a non-conductive nanoporous template has to be created and then nanostructures are formed by electrodeposition into the template which is coated with a metal on one of its sides or placed on a metal surface.
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Thermostat for system testing at low temperatures
Sedláček, Jiří ; Hrdý, Radim (referee) ; Hubálek, Jaromír (advisor)
This bachelor thesis deals with proposal and construction of device that should serve for testing of developed electronic circuits at lower temperatures than the ambient temperature in laboratory. Thermoelectric Peltier’s coolers are used for cooling. The temperature regulation is provided by microcontroller control. The thesis is divided into three parts. First part includes necessary theory for realization of the device. In second part there is the detailed description of construction and function of the device. The third part summarises the measured parameters of the device. The thesis includes the technical documentation.
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