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Comparison of PCR methods suitable for identifikation of yeasts in foodstuffs.
Zovčáková, Monika ; Márová, Ivana (referee) ; Vránová, Dana (advisor)
This bachelor´s thesis deals with identification of yeasts by PCR method in foods. It includes basic information about yeast´s cell. There are elaborated molecular methods of yeast identification. Yeasts are crucial agents in many important natural and industrial bioprocesses as well as in spoilage of foods. Classical methods include morphological and biochemical tests, which are time consuming. The rapid detection and identification of yeasts is necessary for assessment of their beneficial and harmful roles in the production and spoilage of foods.
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L-ascorbyl-6-palmitate and its utilization in dietary and cosmetic supplements.
Palíková, Petra ; Zemanová, Jana (referee) ; Vránová, Dana (advisor)
Bachelor‘s thesis deals with the effection of antioxidants in foods and cosmetics supplements. Some antioxidants as vitamin A and beta-carotene, vitamin E and tocopherols and vitamin C as L-ascorbic acid and her derivate L-ascorbate-6-palmitate are described here. This derivate can by used as an accessory material in cosmetics and foods preparations, especially in the preparations that contain oil or fats, because as a lipophilic compound keeps them from getting rancid.
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Effective components of dietary supplements and their relation to treatment of illness.
Brummer, Vladimír ; Zemanová, Jana (referee) ; Vránová, Dana (advisor)
This bachelor’s thesis is aimed at effective components of dietary supplements in relation to prevention and treatment of civilization illnesses. In thesis are defined basic terms as food supplement, dietary supplement and more. In particular chapters are described characteristics of civilization illnesses and metabolic, physiologic and other changes that are coincidental sign of these diseases. Mentioned are also risk factors of illnesses and summarized dietary and other recommendations to prevent outbreak of disease, or to delay its formation. To each civilization disease are assigned dietary supplements that were studied as prospective effective compounds in prevention and cure of these illnesses. The goal was to summarize and well-arrange available informations related to given theme from various information sources.
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Possibilities for utilization of selected vegetable oils in foodstuffs.
Krátká, Milena ; Vránová, Dana (referee) ; Zemanová, Jana (advisor)
The aim of this work was to find out and compare the composition of the chosen vegetable oils with regards to both the chemical composition and the pharmacological effects as the supplementary nutrition. All the oils selected contain large amount of the essential fatty acids, which has the positive effect on human health. Evening Primrose oil, borage oil and black currant seeds oil contain the large amount of the gama-linolenoic acid (GLA), which has the positive influence on the curing of the diabetes, menstrual syndrome, cancer, cardiovascular diseases, viral infections and skin disorders. Flax and hemp oil are excellent sources of the essential fatty acids in optimal ratio and are rich in other important substances, like phytoestrogenes and phytosterols. The lipids containing the high amount of the unsaturated fatty acids are predisposed to the oxidation during the storage. The oxidized essential fatty acids have the antimetabolic effect, which is working against the positive effect of original essential fatty acid. It is thus important and recommended to accompany the consumption of the polyunsaturated fatty acids (PUFA) with the antioxidants. In this work the effects of the antioxidants were also taken into account. From the oils selected the highest amount of the antioxidants can be found in Sea buckthorn oil, which contains the antioxidants like vitamin E, carotenoids etc. This work was also aimed at the literature review of the stabilization of the unsaturated fatty acids, technological production processes, reactions taking place in oils during their storage and the analytical methods used for the characterization of the oils.
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Active ingredients in sun cosmetics
Jochimová, Eva ; Vránová, Dana (referee) ; Zemanová, Jana (advisor)
Active substances in sun cosmetics mean quantity and properties of UV-filters that absorb rays in UVA or in UVB areas. In the United States of America there are they considered as pharmaceuticals, however in European Union are they taken behind cosmetics. Their usage is very important, because the sun radiation can be very dangerous. Hazard of negative influences increases largely by downgrade of atmosphere and above all by enlargement of ozone hole. UVB and UVA rays penetrate into the skin where they can be a cause of different disorders. They evocate simply as far as heavily curable photodermatosis, skin ageing and cutaneous carcinoma in worse case.
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Fruit utilizing occasion of less knowing bushes
Mařáková, Veronika ; Vránová, Dana (referee) ; Vespalcová, Milena (advisor)
This bachelor thesis deals with cornelian cherry (Cornus mass). The beginning of the thesis treats with botanical characteristics of cornelian: description of the plant, its expansion, reproduction and location. There are described selected species, agricultural outplanting of the cornelian, its harvest and categorization to market kinds according to regulation of Ministry of Agriculture. Attention is given to actual substances contained in its fruits, especially vitamin C, which is presented in high amount there. This shrub includes many other important substances, like natural dyes - anthocyanins, minerals, organic acids, proteins, pectines, sugars, pulp and others. Fresh fruits are used mainly in food and cosmetic industry. At the end there are mentioned methods for determination of vitamin C by high performance liquid chromatography. The other possible ways how to determine vitamin C are titration method, spectroscopical method, polarographic method and gas chromatography.
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Thermophilic microorganisms
Sůkalová, Kateřina ; Vránová, Dana (referee) ; Babák, Libor (advisor)
This bachelor’s thesis is focused on distribution thermophilic species from among microorganisms, characteristic important genera, in which these microorganisms we can find and detailed description choice species. There is described origin of thermal environment and locations of habitation thermophilic microorganisms with intention on geothermal areas, where is found the biggest number of these species. In consequence of thermal stability of enzymes these microorganisms are used in food industry, in waste treatment, in medicine and for their production of thermostabile enzymes.
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The relationship between kidney stone formation and the composition of food.
Zbořilová, Eliška ; Márová, Ivana (referee) ; Vránová, Dana (advisor)
This bachelor’s thesis deals with causes of formation and identification of urinary concrements. The theoretical part of this study includes basic definition of problems with urolithiasis, points to risk factors of formation of urinary concrements and deals with their classification in accordance to their chemical composition. Important part of this study has been devoted to dietary habits, because composition of ingested food and daily intake of liquids is very important for formation of concrement in urinary tract. Analysis of urinal concrement and following identification of its composition is absolutely unavoidable for definition of subsequent therapeutic method of treatment of urolithiasis. In the experimental part, infrared spectroscopy has been used for analysis of urinal concrements. The calibration lines of the most frequent two-component mixtures of urinary concrements have been scaled by this method
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