Zovčáková, Monika - Výsledky hledání - Digitální repozitář

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	Identification of probiotic Bifidobacterium strains in dairy products Zovčáková, Monika ; Horák, Daniel (oponent) ; Španová, Alena (vedoucí práce) Lactobacilli are dominant bacteria of the vaginal flora. Lactobacillus-containing probiotics products are used for the treatement and profylaxis of bacterial urogenital infections. This work is focused on DNA identification and species identification of probiotic bacteria in 5 different vaginal tablets using molecular-genetic methods. Total DNA isolated from complex matrix of vaginal tablets was used for amplification in polymerase chain reaction. DNA was isolated from crude cell lysates by magnetic particles P(HEMA-co-GMA) and by method of phenol extraction. Identification of species of probiotic bacteria was verified using genus-specific and species-specific PCRs. Results of bacterial identification obtained by PCR were compared with declared specification given by producers. Bacteria of genus Lactobacillus were proved in all tablets whereus species identification was in accordance with the stated composition in 1 tablet only. Úplný záznam
	Identification of probiotic Bifidobacterium strains in dairy products Zovčáková, Monika ; Horák, Daniel (oponent) ; Španová, Alena (vedoucí práce) Lactobacilli are dominant bacteria of the vaginal flora. Lactobacillus-containing probiotics products are used for the treatement and profylaxis of bacterial urogenital infections. This work is focused on DNA identification and species identification of probiotic bacteria in 5 different vaginal tablets using molecular-genetic methods. Total DNA isolated from complex matrix of vaginal tablets was used for amplification in polymerase chain reaction. DNA was isolated from crude cell lysates by magnetic particles P(HEMA-co-GMA) and by method of phenol extraction. Identification of species of probiotic bacteria was verified using genus-specific and species-specific PCRs. Results of bacterial identification obtained by PCR were compared with declared specification given by producers. Bacteria of genus Lactobacillus were proved in all tablets whereus species identification was in accordance with the stated composition in 1 tablet only. Úplný záznam
	Comparison of PCR methods suitable for identifikation of yeasts in foodstuffs. Zovčáková, Monika ; Márová, Ivana (oponent) ; Vránová, Dana (vedoucí práce) This bachelor´s thesis deals with identification of yeasts by PCR method in foods. It includes basic information about yeast´s cell. There are elaborated molecular methods of yeast identification. Yeasts are crucial agents in many important natural and industrial bioprocesses as well as in spoilage of foods. Classical methods include morphological and biochemical tests, which are time consuming. The rapid detection and identification of yeasts is necessary for assessment of their beneficial and harmful roles in the production and spoilage of foods. Úplný záznam

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