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The popularity of the sport among the general public in relations to its presentatiton on TV
Trnková, Lucie ; Čáslavová, Eva (advisor) ; Štědroň, Bohumír (referee)
Title: The popularity of the sport among the general public in relations to its presentation on TV Objectives: The aim of this thesis is to determine the popularity of the sport among the general public and to compare it with its presentation on TV. The popularity and viewership of the sport is determined by questioning the public. To conduct interviews with experts on sports broadcasters from television, explaining views of sport news provided on public and commercial televisions. Final results were compared with the results of observation presentation of the sport in sport news in the thesis of Trnková (2014). The results allowed to propose recommendations which will be for use in marketing, sports and media organisations. The results will only imply a certain degree of scientific contribution. Methods: The market research of this thesis has consisted of quantitative and qualitative methods. An electronic questionnaire published on the server Vyplňto.cz, helped gather results from the public. Interviews were carried out in-depth including electronic questioning via e-mail correspondence. The theoretical part is based on the analysis of documents. Results: Sport is very popular within the Czech population in its active and passive form. Based on research carried out, men are more interested in...
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Presentation of sports in television broadcasting in the Czech Republic
Trnková, Lucie ; Čáslavová, Eva (advisor) ; Šíma, Jan (referee)
Title: Presentation of sports in television broadcasting in the Czech Republic Objectives: The aim of this thesis is to measure and compare the airtime devoted to individual sports and sports representations on public and commercial television in the regular, prime-time sports news. The recommendations will be proposed to be used in sports-club marketing, based on the results of observations. Methods: This thesis uses, as a method of observation, the standard sport news shown at prime-time on the TV stations ČT1 and TV Nova. This is the type of indirect observation with the technical devices - USB TV tuner and PC. In terms of content, Berelson's quantitative content analysis will be used, which examines the content of media messages. The theoretical part of the thesis is based on an analysis of documents. Results: The results of observations illustratively show the different approaches between public and commercial television for the inclusion of sport in the broadcasting of regular sports news in prime-time. For both channels, the most popular sports in the sports news are football and ice hockey. In addition, there is a trend of popularity of summer sports, even in the winter time. Sports broadcasting has high frequency of airtime on public Czech television broadcasting. TV Nova focuses on the...
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Modification of glutathione S-transferase by selected glycating agents
Průchová, Zuzana ; Dršata, Jaroslav (advisor) ; Trnková, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Zuzana Průchová Supervisor: Prof. MUDr. Jaroslav Dršata, CSc. Title of diploma thesis: Modification of Glutathione S-transferase by selected glycating agents Glycation is a non-enzymatic reaction, which leads to the binding of carbonyl compounds to free amino groups of amino acids, peptides, proteins or other biomolecules. This process is closely related to impaired protein function in diabetes mellitus, during aging, and in neurodegenerative diseases. The main purpose of this thesis was to monitor the impact of several glycating agents on the properties of the enzyme glutathione S-transeferase (GST, EC 2.5.1.18) which plays a key role in the detoxification of many exogenous as well as endogenous compounds. Methylglyoxal (0.5-2 mM), glyceraldehyde (0.5-10 mM), glucose (50 mM), and fructose (50 mM) were selected as glycating agents. The course of protein glycation was evaluated by following methods: GST catalytic activity assessment, determination of primary amino groups, assessment of AGEs formation using fluorescence and Western blotting, generation of high molecular cross-links and aggregates by denaturationg polyacrylamide gel electrophoresis (SDS-PAGE), changes in GST molecular...
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Interaction of Proteins with Low-molecular Substances in vitro. Effect of Glycation, Substances of Plant Origin, and Combination of these Factors on Function and Spectral Properties of Selected Proteins.
Trnková, Lucie ; Dršata, Jaroslav (advisor) ; Patočka, Jiří (referee) ; Mikšík, Ivan (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate Ing. Lucie Trnková Supervisor Prof. MUDr. Jaroslav Dršata, CSc. Title of Doctoral Thesis Interaction of proteins with low-molecular substances in vitro. Effect of glycation, substances of plant origin, and combination of these factors on function and spectral properties of selected proteins. Plant polyphenolic compounds naturally occurring in human diet possess a wide range of biological and pharmacological properties. They can interact with various proteins including enzymes. Their interaction with serum albumin has a great significance because of its ability to bind, transport and store many endogenous and exogenous low-molecular compounds present in blood circulation. One of the objectives of this thesis was to study chemical structure-binding affinity relationships of two important groups of polyphenolic substances, namely hydroxycinnamic acids and catechins (flavanols), with the molecule of serum albumin in the in vitro models using spectroscopic (UV-Vis absorption spectroscopy, fluorescence quenching method) and electrophoretic (native and SDS PAGE) methods. Some polyphenols caused the changes in protein conformation and the relationships between their structures and obtained...
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Modification of glutathione S-transferase by selected glycating agents
Průchová, Zuzana ; Dršata, Jaroslav (advisor) ; Trnková, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Zuzana Průchová Supervisor: Prof. MUDr. Jaroslav Dršata, CSc. Title of diploma thesis: Modification of Glutathione S-transferase by selected glycating agents Glycation is a non-enzymatic reaction, which leads to the binding of carbonyl compounds to free amino groups of amino acids, peptides, proteins or other biomolecules. This process is closely related to impaired protein function in diabetes mellitus, during aging, and in neurodegenerative diseases. The main purpose of this thesis was to monitor the impact of several glycating agents on the properties of the enzyme glutathione S-transeferase (GST, EC 2.5.1.18) which plays a key role in the detoxification of many exogenous as well as endogenous compounds. Methylglyoxal (0.5-2 mM), glyceraldehyde (0.5-10 mM), glucose (50 mM), and fructose (50 mM) were selected as glycating agents. The course of protein glycation was evaluated by following methods: GST catalytic activity assessment, determination of primary amino groups, assessment of AGEs formation using fluorescence and Western blotting, generation of high molecular cross-links and aggregates by denaturationg polyacrylamide gel electrophoresis (SDS-PAGE), changes in GST molecular...
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Influence of hydroxycinnamic acids on methylglyoxal-induced protein glycation
Vejvalková, Hana ; Boušová, Iva (advisor) ; Trnková, Lucie (referee)
Author: Hana Vejvalková Publishing place: Charles University in Prague, Faculty of Pharmacy in Hradec Králové Title: Influence of hydroxycinnamic acids on methylglyoxal-induced protein glycation Supervisor: PharmDr. Iva Boušová, Ph.D. The diploma thesis was aimed at potencial antiglycation effect of antioxidants of natural origin (hydroxycinnamic acids) on the modification of bovine serum albumine and asparate aminotransferase by methylglyoxal as a glycating agent. I monitored the effect of hydroxycinnamic acids on generation of glycation products using fluorescent, absorption, and electrophoretic methods. The influence of hydroxycinnamic acids on the glycation of model proteins was ambiguous. The only derivative with proven antiglycation effect on production of non-specific glycation products and pentosidin measured by fluorescence and spectrophotometry was p-coumaric acid in 100 μM concentration. On the other hand, chlorogenic acid 100 μM displayed strong proglycation effects in all methods used for detection of glycation products. The effect of all tested substances was concentration dependent. Results show that the more substituents given acid contained in its molecule, the stronger proglycation effect this compound exerted.
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Dotační možnosti realizace rozvoje cestovního ruchu v mikroregionu Horňácko
Trnková, Lucie
This thesis evaluates the use of grant funds from various grant schemes for transnational, national, regional and local level in relation to support the development of tourism. The chosen problem area for the evaluation is the micro-region Horňácko. Review of the literature explains the concepts related to the evaluation of strategic documents, programs, projects, contemporary approaches and documentation as well as methods for the evaluation. The thesis also analyzes the main strategic documents related to the development of the monitored area through which the importace of development is confirmed. Especially in the area of tourism there is a potential area specific for such development. There are also included characteristics of the area, history and explanation of the specific municipalities. The usage of financial aids for the development of tourism is evaluated in the analysis of realized projects. Various projects in the field of tourism development in Horňácko are specialy evaluated with objectives of strategic documents and the usage of potential in the area. In the evaluation we find essential persistent problems which are confirmed by the content of the new LAG strategy for the next programming period from 2014 to 2020. There are researched further possibilities of financial aid for new programming period from several sources availible on different levels in the framework of ascertined facts.
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The European Union and phenomenon of migration - Slovenian case
TRNKOVÁ, Lucie
On the beginning of the diploma thesis the student will deal with the issue of migration in general terms, brief explanation of basic terms also from the internatioal law point of view will be presented. Diploma thesis first addresses the phenomenon of migration in general, briefly explains the basic concepts and typology. After this theoretical definition the focus shifts to more practical plane, focusing on selected global migration trends that are currently taking place within the European Union, and approaches to international law on immigration issue, including the European Union's attempt to create a common asylum and migration policy . The final part of the thesis is essentially an analytical study of migration issues on the territory of Slovenia, a relatively prospective member of the European Union with {\clq}qBalkan" history, and his position due to the shaping of their own at first, then Europe's asylum and migration policy.
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