|
|
Spectroscopic study of key biomolecular interactions for therapeutic applications of modified oligonucleotids
Kříž, Martin
Title: Spectroscopic study of key biomolecular interactions for therapeutic applications of modified oligonucleotides Author: Martin Kříž Department: Institute of Physics of Charles University Supervisor: Prof. RNDr. Josef Štěpánek, CSc., Institute of Physics of Charles University Abstract: This dissertation thesis is focused on study of biomolecular interactions that are crucial for therapeutic applications of modified oligonucleotides. Interactions of nucleic acids, in particular hybrid DNA: RNA duplexes with fully complementary sequences, as well as DNA: RNA duplexes with a central mismatch were studied. The molecular model was created. With this model the influence of five different chemical modifications in the DNA strand to its binding ability was studied. Structural changes were characterized by Raman spectroscopy using a precise differential and double differential spectra. These data were supplemented by thermodynamic parameters computed from melting curves obtained by UV absorption spectroscopy. Furthermore, the Raman spectroscopy was applied on study of conformation changes in the RNase L protein. These changes in the conformation occur after ligand binding, which in this case, either natural or modified short oligonucleotide. Measurement of RNase L was performed using a special experimental...
|
|
|
Diversity, phylogeny and ecology of members of the family Geastraceae
Zehnálek, Petr ; Koukol, Ondřej (advisor) ; Kříž, Martin (referee)
The family Geastraceae is a group of Fungi with global distribution and with saprophytic manner of nutrition. Its members have basic characters of class Gasteromycetes, which no longer exists. The fruitbodies have angiocarpous morphology and peridia are well developer and very distinct in their functions. Star-shaped mature fruitbody is the most typical character of these interesting mushrooms. This bachelor's thesis deals with family Geastraceae. Its object is give a basic overview about the actual state of knowledge of this group of Fungi. It is group that is known from all continets besides from Antarctica. The members of family Geastraceae are saprophytes and they are found in different forest and non-forest habitats. The members of this familia are characterized by their morphology with a special terms and it will be described in details. Other aim is a recapitulation historical development family Geastraceae in system of Basidiomycota. Several recent molecular phylogenetic studies brought some new surprising knowledges about taxonomy of the family Geastraceae and the membership of some genera was questioned.
|
|
|
Spectroscopic study of key biomolecular interactions for therapeutic applications of modified oligonucleotids
Kříž, Martin ; Štěpánek, Josef (advisor) ; Bednárová, Lucie (referee) ; Mašek, Vlastimil (referee)
Title: Spectroscopic study of key biomolecular interactions for therapeutic applications of modified oligonucleotides Author: Martin Kříž Department: Institute of Physics of Charles University Supervisor: Prof. RNDr. Josef Štěpánek, CSc., Institute of Physics of Charles University Abstract: This dissertation thesis is focused on study of biomolecular interactions that are crucial for therapeutic applications of modified oligonucleotides. Interactions of nucleic acids, in particular hybrid DNA: RNA duplexes with fully complementary sequences, as well as DNA: RNA duplexes with a central mismatch were studied. The molecular model was created. With this model the influence of five different chemical modifications in the DNA strand to its binding ability was studied. Structural changes were characterized by Raman spectroscopy using a precise differential and double differential spectra. These data were supplemented by thermodynamic parameters computed from melting curves obtained by UV absorption spectroscopy. Furthermore, the Raman spectroscopy was applied on study of conformation changes in the RNase L protein. These changes in the conformation occur after ligand binding, which in this case, either natural or modified short oligonucleotide. Measurement of RNase L was performed using a special experimental...
|
|
|
New Diagnostic Methods in Dermatology: Non-invasive Diagnosis of Non-melanoma Skin Cancer
Kříž, Martin ; Arenberger, Petr (advisor) ; Pavel, Stanislav (referee) ; Vašků, Vladimír (referee)
Non-melanoma skin cancer (NMSC) is the most prevalent cancer in the light-skinned population. New less- or non-invasive treatments are increasingly used for NMSC patients, making the development of non-invasive diagnostic technologies highly relevant. The scope of this work is to present data on the current state-of-art in diagnostic methods for NMSC and actinic keratosis. Different key words were searched through scientific search-engines. Results of the two of our studies are presented. Encouraging results from the reviewed diagnostic trials have suggested a high diagnostic accuracy for many of the new technologies. New imaging technologies have the capability of providing new or additional data or enough information in vivo to understand of NMSC development and to help the clinical diagnostics. Some of the new technologies, especially the high frequency ultrasonography described in the paper have the potential to be used in the daily praxis of NMSC diagnostic. The fluorescence diagnostic brings in our point of view a great merit in second line diagnostics of advanced and not clear cases.
|
|
| |
|
| |
|
|
Language and style of Chinese propaganda
Kříž, Martin ; Lomová, Olga (advisor) ; Sehnal, David (referee)
Na základě provedených rozborů textů jsem určil některé typické znaky jazyka totalitní moci, které se objevují v jazyce agitátorů Komunistické strany Číny. Některé z nich odpovídají charakteristikám, kterými se vyznačuje LTI a jazyk komunistické propagandy ČSSR a SSSR a kterými se ve svých analýzách zabývali Victor Klemperer a Petr Fidelius. Jiné prvky jazyka propagandy Komunistické strany Číny jsou jedinečné a odrážejí hlavně kulturní odlišnosti. Největším tématem textů podrobených analýze byla Komunistická strana Číny a její úloha. V tomto směru se autoři textů občas dostali i k významu socialismu. V počátcích Čínské lidové republiky se kladl velký důraz na úlohu komunistické strany při založení republiky. Straně patřil v tomto směru všechen dík, neboť ta jediná se zasloužila o osvobození čínského lidu, sjednocení země a založení republiky. Strana byla odpovědná i za budoucnost lidu, neboť lid vedla a stanovovala cíle, které měly zem dovést ke světlým zítřkům. Ve spojení s počátky Čínské lidové republiky se také v textech objevil první, čistě čínský termín ,.demokratická diktatura lidu'' respektive "lidovědemokratická diktatura" (renmin minzhu zhuanzhenK Á !0 fl! i:' i")Jfr). Autorem nebyl nikdo jiný než "Velký kormidelník" Mao Zedong. V tomto případě jsem došel k závěru, že se jedná o změnu významu na...
|
|
|
Relaxed rebalancing of binary search trees
Kříž, Martin ; Koubek, Václav (referee) ; Koubková, Alena (advisor)
In standard binary trees the rebalancing is carried out in connection with and immediately folowing the updates. Relaxed balancing allows to separate updates and rebalancing. First advantage of this approach is to keep the tree partially unbalanced and leave rebalancing to the different time when system is idle. Other great benefit of presented relaxed balancing algorithms in concurrent enviroment is necessity of keeping only small constant number of locks for modifying operations and thus allowing more modifying operations in the tree at the same time. The aim of this thesis is to empirically compare standard and relaxed variant of AVL tree in several different scenarios in concurrent enviroment according to number of data compares, number and type of rotations and according to the time requirements.
|
|
|
Chinese Economic System: Market Economy or State Capitalism?
Bejkovský, Jan ; Jiránková, Martina (advisor) ; Stuchlíková, Zuzana (referee) ; Fárek, Jiří (referee) ; Kříž, Martin (referee)
This dissertation deals with the nature of the Chinese economic system. The first chapter focuses on the global economy and seeks to define the main economic systems prevailing in it, ie. Anglo-Saxon, Continental and Asian-developmental. Compared to these systems defined as market economies, the dissertation consequently analyses and defines a system of state capitalism and the main elements of it. The following part of the dissertation is focused on the historical Chinese approaches to economic governance of the country and attempts to identify those historical approaches that are still relevant and influential in modern Chinese economy. The third chapter analyses the Chinese economic system and the role of the state in it; it also examines the government's approach to the ownership of state enterprises and the overall extent of state ownership in the Chinese economy. The final chapter examines the different types of state subsidies to domestic, often state, enterprises in China. As an example of the various subsidies a case study on the automotive industry is used.
|
|
|
The Proposal of Taxation Incomes Legislation Nonnursed Enterprise in the Czech Republic
Kříž, Martin ; Cabala, Martin (referee) ; Kopřiva, Jan (advisor)
The Bachelor thesis contains two parts, the theoretical part and the practical part. In the theoretical part of thesis is deal with brief history of prostitution, it’s developement as in europian measure so as in the territory of present land of Czech Republic, also deal with current situation of legally-taxation treatment. The practical part of thesis is focus on comparation of mention threatment with another europian countries and at the same time suggest how the new threatment can look like or how the old one might be innovated.
|
guest ::
