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Voltammetric Determination of Genotoxic 2-Nitrofluorene at Screen-Printed Carbon Electrodes
Jelínková, Lenka ; Vyskočil, Vlastimil (advisor) ; Zima, Jiří (referee)
of the Bachelor Thesis Voltammetric behavior of genotoxic 2-nitrofluorene (NF) has been investigated using direct current voltammetry (DCV) and differential pulse voltammetry (DPV) at a screen- printed carbon electrode (SPCE). The optimum conditions have been found for its determination in a concentration range from 1×10−6 to 1×10−5 mol l−1 for DCV technique and from 1×10−7 to 1×10−5 mol l−1 for DPV technique. An optimum medium for DCV at the SPCE was a 0.1 mol l−1 acetate buffer of pH 4.6, an optimum time of stirring of the sample prior to the analysis was 120 s (the attained limit of quantification (LQ) was 8.5×10−6 mol l−1 of NF). For DPV at the SPCE, the optimum medium was an acidic component part of the Britton - Robinson buffer of pH 2.0; the attained LQs were 6.2×10−7 mol l−1 of NF for the concentration range from 1×10−6 to 1×10−5 mol l−1 (at the stirring time of the sample of 120 s) and 2.0×10−7 mol l−1 of NF for the concentration range from 1×10−7 to 1×10−6 mol l−1 (at the stirring time of the sample of 300 s).
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The role of innate immunity cells in the pathogenesis of celiac disease
Dáňová, Klára ; Palová Jelínková, Lenka (advisor) ; Černý, Jan (referee)
Celiac disease is an autoimmune disease which occurs in susceptible individuals after ingestion of food containing gluten. Gluten and its monomeric fraction gliadin induce inflammatory damage of the small intestine by activating the immune cells that react strongly to gluten peptides. Gluten peptides have the ability to activate cells of adaptive as well as innate immune system. This work is focused on the production of interleukin (IL)-1 in antigen presenting cells stimulated with peptic gliadin digest. We found that monocytes and peripheral blood mononuclear cells (PBMC) isolated from blood of celiac patients secrete significantly more IL-1α and IL-1β than cells of healthy donors after stimulation with gliadin digest. The gliadin-induced IL-1β expression is controlled by a signaling cascade that includes MAPK kinase family molecules and transcription factor NF-κB. Moreover, we found that the adaptor proteins MyD88 and TRIF as well as Toll-like receptor (TLR) 2 and 4 play a role in the signaling cascade underlying gliadin-induced IL-1β expression by using murine bone marrow derived dendritic cells (BMDC). The precursor form of IL-1β in gliadin- stimulated PBMC and murine BMDC is maturated by caspase-1. In celiac PBMC the gliadin- induced maturation and secretion of IL-1β depends on the potassium...
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Problems of description of electronic online resources in contemporary library cataloguing practice
Jelínková, Lenka ; Bratková, Eva (advisor) ; Drobíková, Barbora (referee)
The topic of the thesis is bibliographic description of electronic online resources in foreign and Czech contemporary cataloguing practice. The first part of the thesis analyzes the development of description of electronic online resources in 90th of the 20th century along with an introduction of important foreign projects which contributed to this development. The paper analyzes in detail all areas of bibliographic description according to ISBD(ER) and the fields of MARC 21 format used for description of electronic online resources. The thesis also evaluates cataloguing practice of the National library of the Czech Republic within WebArchiv project and building of subject gateways. The heart of the thesis is detailed analyze of illustrating examples of description of electronic online resources including cataloguing records with comments clarifying some difficult questions of desciption of this resources. Final part of the thesis analyzes alternative approaches in the field of bibliographic description of electronic online resources, escpecially metadata formats and their most widespread representative within library community - Dublin Core. The thesis deals also with possible future perspectives of cataloguing practice and shortly introduces new cataloguing standard RDA.
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Bibliographic description of electronic online resources in foreign and Czech cataloguing practice
Jelínková, Lenka ; Bratková, Eva (advisor) ; Celbová, Ludmila (referee)
Tématem diplomové práce je bibliografický popis elektronických online zdrojů z pohledu zahraniční a domácí katalogizační praxe. V úvodu práce je nastíněn vývoj popisu elektronických online zdrojů v průběhu 90. let minulého století spolu s představením významných zahraničních projektů, které k tomuto vývoji přispěly. Dále jsou podrobně analyzovány jednotlivé oblasti popisu dle standardu ISBD(ER) a jednotlivá pole formátu MARC 21 pro popis elektronických online zdrojů. Zhodnocena je rovněž katalogizační praxe Národní knihovny ČR v rámci projektu WebArchiv a oborových informačních bran. Jádrem práce je podrobná analýza ukázkových příkladů popisu elektronických online zdrojů obsahující katalogizační záznamy spolu s komentáři objasňujícími sporné otázky popisu těchto zdrojů.
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Online marketing of chosen company
Jelínková, Lenka ; Pilař, Ladislav (advisor) ; Poláková, Jana (referee)
The subject of the bachelor thesis is a summary of selected marketing tools of chosen company that operates on the market in the production and sale of plastic castings. Subsequently, the plan of online marketing activities is made. The first part focuses on the theoretical basis. The comparison and the description of scientific literature are used to define core concepts. They are also an essential foundation for creating practical part. The theoretical part explains concepts like marketing and to it associated marketing mix and its components, marketing communication is described in more details, strategic management and Internet marketing. The second part, the practical part, was written using other sources. All the elements served to create a plan of chosen company. The plan is created with the help of the description of the current situation and goals that the company would like to achieve by means of the strategy and online marketing tools. In the parts of work called the findings and recommendations are itemized suggestions arising from the practical part, which were made during the analysis, observation and drafting of the plan. It builds on them to conclude which is the overall summary of the discussed issues. Bachelor work is created for a purpose that the proposed plan could be apllied in the following months.
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Development of drawing of a human body of a pre-school child
JELÍNKOVÁ, Lenka
This bachelor thesis is focused on preschool child conception of human figure. The theoretical part is about art imaging of preschool children. It also deals with its relationship with the development of cognition, perception, graphomotor skills and changes in art imaging development during preschool period. The practical part is based on the assumption that there is a development of preschool children´s drawing of a human figure in a long-term period. The research part of this bachelor thesis is focused on finding typical features of human figure conception that was made by preschool children. This thesis also describes using of newly added features in drawing of a human figure.
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Political business cycle and public procurements at regional authorities
Jelínková, Lenka ; Pavel, Jan (advisor) ; Holubářová, Jana (referee)
This bachelor thesis examines the political business cycle at public procurements, which were submitted by 13 regional authorities in the Czech Republic from September 2004 till June 2010. Two hypotheses are tested here. According the first one, the incumbents try to increase the probability of re-election by visible investments, e.g. public procurements for buildings. The second hypothesis supposes that the incumbents are afraid of not being re-elected. Therefore they strive for maximizing their personal profit from the office. Regression analysis is used for testing. Public procurements for buildings are tested separately from public procurements for goods and services. The number of public procurements, total amount and the average number of bidders are tested for each group of public procurements. The result is a detection of different motives in different parts of political business cycle.
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Účast monocytů a dendritických buněk v patogenezi celiakie
Tučková, Ludmila ; Palová-Jelínková, Lenka ; Rožková, D. ; Černá, M. ; Pecharová, Barbara ; Smythies, L. ; Smith, P. ; Dvořák, M. ; Fruhauf, P. ; Tlaskalová, Helena
This work studies the involvement of monocytes and dendritic cells in coelias cisease pathogenesis
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