guest ::
| Home > Conference materials > Papers > Expression of osmotin, an antifungal protein from Nicotiana tabacum in Escherichia coli |
Original title:
Expression of osmotin, an antifungal protein from Nicotiana tabacum in Escherichia coli
Authors: Viktorová, J. ; Macková, M. ; Macek, Tomáš
Document type: Papers
Conference/Event: Biologically Active Peptides /12./, Praha (CZ), 2011-04-27 / 2011-04-29
Year: 2011
Language: eng
Abstract: Plants have evolved a huge variety of proteins involved in the defense against pathogens and adaptation to stressful environments. Plant proteins whose expression is strongly induced in response to infection by pathogens belong to the group of pathogenesis-related (PR) proteins. The family of PR-5 proteins constitutes a group of cysteine-rich proteins including thaumatin, zeamatin and also osmotin. Osmotin is a cationic protein of 205 residues and molecular weight of 24 kDa. It was discovered and characterized in cells of Nicotiana tabacum var. Wisconsin 38. The plasmid harbouring cDNA of osmotin from Nicotiana tabacum was constructed for transformation of Escherichia coli. The osmotin gene was prepared in fusion with histidine tail to facilitate the isolation and purification from bacterial cells. Selection of transgenic colonies was based on antibiotic resistance. The hexahistidine-tagged osmotin was overexpressed in heterologous system by using pET expression vector and purified using immobilized metal affinity chromatography. The expression of osmotin was detected and antifungal activity was tested.
Keywords: antifungal activity; osmotin; pathogenesis-related proteins; recombinant protein
Project no.: CEZ:AV0Z40550506 (CEP), GAP501/11/1654 (CEP)
Funding provider: GA ČR
Host item entry: Biologically Active Peptides. 12th Conference, ISBN 978-80-86241-44-9
Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0204379
Permalink: http://www.nusl.cz/ntk/nusl-81034
The record appears in these collections:
Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers
Authors: Viktorová, J. ; Macková, M. ; Macek, Tomáš
Document type: Papers
Conference/Event: Biologically Active Peptides /12./, Praha (CZ), 2011-04-27 / 2011-04-29
Year: 2011
Language: eng
Abstract: Plants have evolved a huge variety of proteins involved in the defense against pathogens and adaptation to stressful environments. Plant proteins whose expression is strongly induced in response to infection by pathogens belong to the group of pathogenesis-related (PR) proteins. The family of PR-5 proteins constitutes a group of cysteine-rich proteins including thaumatin, zeamatin and also osmotin. Osmotin is a cationic protein of 205 residues and molecular weight of 24 kDa. It was discovered and characterized in cells of Nicotiana tabacum var. Wisconsin 38. The plasmid harbouring cDNA of osmotin from Nicotiana tabacum was constructed for transformation of Escherichia coli. The osmotin gene was prepared in fusion with histidine tail to facilitate the isolation and purification from bacterial cells. Selection of transgenic colonies was based on antibiotic resistance. The hexahistidine-tagged osmotin was overexpressed in heterologous system by using pET expression vector and purified using immobilized metal affinity chromatography. The expression of osmotin was detected and antifungal activity was tested.
Keywords: antifungal activity; osmotin; pathogenesis-related proteins; recombinant protein
Project no.: CEZ:AV0Z40550506 (CEP), GAP501/11/1654 (CEP)
Funding provider: GA ČR
Host item entry: Biologically Active Peptides. 12th Conference, ISBN 978-80-86241-44-9
Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0204379
Permalink: http://www.nusl.cz/ntk/nusl-81034
The record appears in these collections:
Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers
Record created 2012-01-11, last modified 2024-01-26