Original title: Study of antimicrobial peptide induction in Brassica napus
Authors: Shevchenko, I. ; Neubauerová, Tereza ; Macková, Martina ; Macek, Tomáš
Document type: Papers
Conference/Event: Biologically Active Peptides /12./, Praha (CZ), 2011-04-27 / 2011-04-29
Year: 2011
Language: eng
Abstract: Development of cheap, effective and environmentally friendly protection of industrial plants is a key problem of mankind, where using of antimicrobial peptides could be a reasonable solution. As a source for isolation of these peptides canola (Brassica napus) was chosen, which was treated by functional analog of salicylic acid – benzothiadiazole (BTH) – to stimulate the immune system. To isolate the responsible antimicrobial peptides, crude buffer extract was obtained. Next steps were precipitation, centrifugation and molecular weight filtration. The further isolation procedure comprised hexane extraction, SPE-C18 (Solid Phase Extraction) to separate hydrophobic and hydrophilic fractions and reversed-phase chromatography. Antimicrobial activity was tested by diffusion method. Fractions were tested on gram-positive and gram-negative bacteria and on yeast Candida scotii.
Keywords: antimicrobial peptides; Brassica napus; induced resistance
Project no.: CEZ:AV0Z40550506 (CEP), GA522/09/1693 (CEP), GD305/09/H008 (CEP)
Funding provider: GA ČR, GA ČR
Host item entry: Biologically Active Peptides. 12th Conference, ISBN 978-80-86241-44-9

Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0204354

Permalink: http://www.nusl.cz/ntk/nusl-81021


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Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers