Název: Attempts on chromatin immunoprecipitation with \kur{C. elegans} nuclear receptor NHR-25
Překlad názvu: Attempts on chromatin immunoprecipitation with \kur{C. elegans} nuclear receptor NHR-25
Autoři: POSPĚCH, Alexandr
Typ dokumentu: Diplomové práce
Rok: 2010
Jazyk: cze
Abstrakt: The aim of the work presented in this thesis was to establish chromatin immunoprecipitation method in our laboratory as a tool to study target genes of the nuclear receptor NHR-25 in C. elegans. Once the method is established, it will be also useful for studies of other DNA binding proteins. ChIP was performed in transiently transfected cells HEK293 and analyzed using PCR and qPCR. Although ChIP is typically used to find authentic target genes in the cell or in organisms, testing protein-DNA interactions by ChIP in transient transfection system (by transfecting both the expression vector of the protein of interest and a vector containing potential binding sequence/promoter of the protein) can be useful as it serves as a relatively quick tool to confirm the direct binding. Since the detection is by PCR, this method is sensitive yet less costly non radioactive method to analyze protein-DNA interaction. For the first step towards ChIP in C. elegans; pulling down tagged protein directly from the worm was also performed as a preparation for in vivo analysis of NHR-25 regulated genes.
Klíčová slova: Caenorhabditis elegans; Chromatin immunoprecipitation; Nuclear receptor
Citace: POSPĚCH, Alexandr. Attempts on chromatin immunoprecipitation with \kur{C. elegans} nuclear receptor NHR-25. České Budějovice, 2010. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Instituce: Jihočeská univerzita v Českých Budějovicích (web)
Informace o dostupnosti dokumentu: Plný text je dostupný v digitálním repozitáři JČU.
Původní záznam: http://www.jcu.cz/vskp/9750

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-80493


Záznam je zařazen do těchto sbírek:
Školství > Veřejné vysoké školy > Jihočeská univerzita v Českých Budějovicích
Vysokoškolské kvalifikační práce > Diplomové práce