Original title: Thermal stability of FAD-dependent oxidoreductase CtAO from thermophilic fungus Chaetomium thermophilum
Authors: Švecová, Leona ; Skálová, Tereza ; Kovaľ, Tomáš ; Ostergaard, L. H. ; Kolenko, Petr ; Malý, M. ; Dohnálek, Jan
Document type: Papers
Conference/Event: Osmá Studentská vědecká konference fyziky pevných látek FJFI ČVUT, Sedliště (CZ), 20180917
Year: 2018
Language: eng
Abstract: Chaetomium thermophilum is a soil-borne thermophilic fungus, which is able to grow in temperatures up to 60 °C. Its thermostable proteins have great potential for usage in high-temperature industry. The Chaetomium thermophilum FAD-dependent oxidoreductase (CtAO) studied here participates in the lignin degradation process, producing hydrogen peroxide for ligninolytic peroxidases. Here we present a study of CtAO thermal stability. The thermal stability was tested in a variety of buffers with pH in range 3.0–9.0. The best results were obtained in the buffer containing 50 mM NaCl and 50 mM Tris-HCl, pH 7.0. In this buffer, CtAO is stable in temperatures up to 65 °C.
Keywords: Chaetomium thermophilum; FAD-dependent oxidoreductase; nano-differential
Host item entry: Sborník Osmé Studentské vědecké konference fyziky pevných látek FJFI ČVUT, ISBN 978-80-01-06511-2

Institution: Institute of Biotechnology AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0307632

Permalink: http://www.nusl.cz/ntk/nusl-669363


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Research > Institutes ASCR > Institute of Biotechnology
Conference materials > Papers