Role of the transcription factor Cytokinin Response Factor 6 (CFR6) in redox signalling

Witkovská, Nora

Cytokinins are phytohormones that mediate many major plant developmental processes but are also recognized for their role in stress tolerance. Recent findings propose that the cytokinin induced transcription factor Cytokinin Response Factor 6 (CRF6) plays an important role in oxidative stress signaling, but the exact mechanism is not yet clear. The main objective of this work was to review the role of CRF6 in stress signaling and to identify novel molecular functions by assessing its protein interactors. By employing single-step immunoprecipitation (IP) and mass spectrometry (MS) analysis of Green Fluorescence Protein (GFP)-labeled CRF6 we identified nearly 300 putative proteins interactors implicated in various functional categories such as metabolism, biosynthesis, signal transduction, ribosome biology, and transport. Interestingly, three major antioxidant enzymes (CATALASE 2 and 3, and ASCORBATE PEROXIDASE 1) were among the putative protein interactors corroborating the idea that CRF6 has a role in modulating redox homeostasis. To validate those findings, we used Yeast Two-Hybrid assay and isolated mutants lacking photorespiratory catalase activity, which can be used to further study the role of CRF6 in stress responses.

host :: přihlásit Digitální repozitář
		Hledej		Nový záznam		Nápověda		O repozitáři