Original title: Enzyme-Based Impedimetric Biosensor dotted with gold nanoparticles
Authors: Košelová, Zuzana ; Fohlerová, Zdenka
Document type: Papers
Language: eng
Publisher: Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologií
Abstract: This research delves into the realm of biosensor improvement through the utilization of gold nanoparticles (Au NPs). The primary objective is to assess the impact of differentsized Au NPs on sensor performance, specifically investigating whether 100 nm or 20 nm nanoparticles prove more favourable to enhancement. Moreover, we aim to inspect the biosensor's response to varied concentrations of Au NPs, unravelling the involved collaboration between nanoparticle size, concentration, and overall sensor properties. This modification of commercial electrodes with Au NPs, could be way for enhancing surface area and enzyme immobilization. Notably, the investigation also explores the potential drawbacks associated with increasing nanoparticle concentration and offers insights into optimizing biosensor design. It has been observed that while 20 nm Au NPs slightly decreased impedance values at higher glucose concentrations, 100 nm Au NPs, conversely, exhibited an increase in capacitive behaviour. Equally crucial is the parameter chosen for constructing the calibration curve. From impedance values at low frequencies of alternating voltage, such as 2 Hz, a lower Limit of Detection (LOD) is obtained. However, the analysis of Rct in the case of 20 nm Au NPs reveals a broader range of glucose concentrations falling within the calibration area. Through a comprehensive analysis of electrochemical behaviour, impedance, and charge transfer resistance, we endeavour to provide contributions to the improvement of biosensor technologies.
Keywords: biosensors; calibration curves; enzyme immobilization; glucose detection; gold nanoparticles; impedance spectroscopy
Host item entry: Proceedings II of the 30st Conference STUDENT EEICT 2024: Selected papers, ISBN 978-80-214-6230-4, ISSN 2788-1334

Institution: Brno University of Technology (web)
Document availability information: Fulltext is available in the Brno University of Technology Digital Library.
Original record: https://hdl.handle.net/11012/249315

Permalink: http://www.nusl.cz/ntk/nusl-622486


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