Vliv dietní intervence na zánětlivou aktivaci nespecifické imunity u pacientů se stabilizovanou celiakií

Selková, Katarína

Introduction: Celiac disease is a complex autoimmune disease induced in genetically predisposed individuals after ingestion of cereals containing gluten. Monocytes, as the main effector cells of innate immunity, play a non-negligible role in the development of the immune response in response to pathogenic and foreign agents as well as in the pathogenesis of celiac disease. The only effective treatment for celiac disease is strict and lifelong adherence to a gluten-free diet, while dietary choices are of key importance in the regulation of this disease. Oats have emerged as a controversial but nutritionally rich option to supplement a gluten-free diet with a source rich in fiber, micro- and macronutrients. Therefore, in this thesis, we focused on how the innate immune system, specifically monocytes, interact with the avenin peptides of a selected gluten-free oat cultivar and whether an immune reaction detectable at the molecular level occurs in response to its consumption. Materials and methods: 39 patients with stabilized celiac disease were included in the study, who consumed 50 g of raw oatmeal of the selected cultivar daily for 14 days. CD14+ monocytes were isolated from whole blood before the start of the study and after its completion using immunomagnetic separation, in which TLR2, TLR4, TNFα,...

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