Original title: Stanovení reziduí antibiotik v půdě
Translated title: Determination of antibiotic residues in soil
Authors: Kaprinay Bréda, Boglárka ; Zlámalová Gargošová, Helena (referee) ; Mravcová, Ludmila (advisor)
Document type: Master’s theses
Year: 2021
Language: slo
Publisher: Vysoké učení technické v Brně. Fakulta chemická
Abstract: [slo] [eng]
Táto diplomová práca je zameraná na optimalizáciu úpravy vzoriek a analytickú metódu pre stanovenie fluorochinolonové látky. V teoretickej časti sú popísané princípy analýzy v pôdnych vzorkách, environmentálne aspekty pri antibiotickej rezistencii, charakteristika metód UPLC-MS/MS, popis chinolónovej látky. Experimentálna časť sa zaoberá identifikáciou a stanovením fluorochinolonové látky (ciprofloxacínu, enrofloxacínu) v pôde, v ktorej bol zapracovaný hnoj z podstielky hydiny za použitia kvapalinovej chromatografia s hmotnostnou spektrometriou (MS/MS, trojitý kvadrupól). Boli používané metódy vnútorného štandardu a štandardného prídavku na kvantifikáciu ciprofloxacínu a enrofloxacínu. Množstvo CIP vo vzorkách sa pohyboval od 0,2030 do 12,7088 µg·g-1 a pre ENR od 0,2537 do 10,0224 µg·g-1. This master‘s thesis is focused on the optimization of sample preparation and analytical method for the determination of fluoroquinolone. The theoretical part describes the principles of analysis in soil samples, environmental aspects of antibiotic resistance, characteristics of UPLC-MS / MS methods, description of quinolone. The experimental part deals with the identification and determination of fluoroquinolone substance (ciprofloxacin, enrofloxacin) in soil in which manure from poultry litter was incorporated using liquid chromatography with mass spectrometry (MS / MS, triple quadrupole). Internal standard and standard addition methods were used to quantify ciprofloxacin and enrofloxacin. The amount of CIP in the samples ranged from 0.2030 to 12.7088 µg·g-1 and for ENR from 0.2537 to 10.0224 µg·g-1.
Keywords: bacterial rezistence; ciprofloxacine; enrofloxacine; extraction; Fluoroquinolones; liquid chromatography; mass spectrometry; soil

Institution: Brno University of Technology (web)
Document availability information: Fulltext is available in the Brno University of Technology Digital Library.
Original record: http://hdl.handle.net/11012/201385

Permalink: http://www.nusl.cz/ntk/nusl-573588


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Universities and colleges > Public universities > Brno University of Technology
Academic theses (ETDs) > Master’s theses