Original title: Využití pilin jako substrátu pro biotechnologickou produkci PHA
Translated title: Utilization of spent wood chips for biotechnological production of PHA
Authors: Ladický, Peter ; Sedláček, Petr (referee) ; Obruča, Stanislav (advisor)
Document type: Bachelor's theses
Year: 2016
Language: cze
Publisher: Vysoké učení technické v Brně. Fakulta chemická
Abstract: [cze] [eng]
Cieľom práce bolo štúdium vhodnosti hoblín a pilín ako substrátu pre mikrobiálnu produkciu PHA baktériami Burkholderia cepacia a Burkholderia sacchari. V experimentálnej časti práce bol skúmaný najvhodnejší spôsob hydrolýzy hoblín a pilín a vplyv koncentrácie furfuralu a polyfenolov na schopnosť akumulovať PHA. Burkholderia sacchari mala v porovnaní s Burkholderia cepacia vyššiu schopnosť akumulovať PHA. Pri kultivácii Burkholderia sacchari na médium obsahujúce detoxifikovaný hydrolyzát z pilín boli dosiahnuté hodnoty kedy PHB predstavoval 87–89 % celkovej koncentrácie biomasy. Piliny teda predstavujú sľubný substrát na mikrobiálnu produkciu PHA z hľadiska zníženia produkčných nákladov a vysokého obsahu PHB v biomase. The aim of this work was to study the suitability of wood shavings and sawdust as a substrate for microbial production of PHA by bacteria Burkholderia cepacia and Burkholderia sacchari. In the experimental part of the work the most appropriate approach of hydrolysis of wood shaving and sawdust and the effect of polyphenol and furfural concentration on ability to accumulate PHA was studied. Burkholderia sacchari had greater ability to accumulate PHA compared to Burkholderia cepacia. PHB values 87–89 % were achieved when Bulkholderia sacchari was cultivated on medium that contained detoxified hydrolysate of sawdust. Sawdust is therefore a promising substrate for microbial production of PHA in terms of reducing production costs and high content of PHB in biomass.
Keywords: Burkholderia cepacia; Burkholderia sacchari; gas chromatography; hydrolysis; P(3HB); PHA; PHB; Polyhydroxyalkanoates; Polyhydroxybutyrate; sawdust; wood shavings; Burkholderia cepacia; Burkholderia sacchari; hobliny; hydrolýza; P(3HB); PHA; PHB; piliny; plynová chromatografia; Polyhydroxyalkanoáty; Polyhydroxybutyrát

Institution: Brno University of Technology (web)
Document availability information: Fulltext is available in the Brno University of Technology Digital Library.
Original record: http://hdl.handle.net/11012/58851

Permalink: http://www.nusl.cz/ntk/nusl-568089


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Universities and colleges > Public universities > Brno University of Technology
Academic theses (ETDs) > Bachelor's theses