Original title:
Tail labelled oligonucleotide probes for the detection of DNA-protein interactions
Authors:
Pivoňková, Hana ;
Němcová, Kateřina ;
Horáková Brázdilová, Petra ;
Havran, Luděk ;
Macíčková-Cahová, Hana ;
Hocek, Michal ;
Fojta, Miroslav
Document type: Papers
Conference/Event: Chemistry of Nucleic Acid Components /15./ , Český Krumlov (CZ), 2011-06-05 / 2011-06-10
Year:
2011
Language:
eng
Abstract:
DNA-protein interactions can be monitored via different ways. We introduce novel, fast and simple approaches in DNA-protein interaction detection based on electrochemical measurements of DNA alone (structure-sensitive DNA sensing), or DNA modified with osmium tetroxide bearing nitrogenous ligands, or measurements of redox-active moieties enzymatically attached to the end of a DNA substrate thus forming a labeled tail (by terminal transferase).
Keywords:
DNA ;
electrochemistry ;
tail labeling
Project no.: CEZ:AV0Z40550506 (
CEP ),
CEZ:AV0Z50040507 (
CEP ),
CEZ:AV0Z50040702 (
CEP ),
LC512 (
CEP ),
LC06035 (
CEP ),
IAA400040901 (
CEP ),
IAA400040903 (
CEP ),
GAP301/11/2076 (
CEP )
Funding provider: GA MŠk, GA MŠk, GA AV ČR, GA AV ČR, GA ČR
Host item entry: Chemistry of Nucleic Acid Components. 15th Symposium, ISBN 978-80-86241-37-1
Institution: Institute of Organic Chemistry and Biochemistry AS ČR
(
web )
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0198898
Permalink: http://www.nusl.cz/ntk/nusl-55850
The record appears in these collections: Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry Conference materials > Papers