Original title: Direct enzymatic synthesis of aldehyde-functionalized DNA and its conjugation with hydrazines and amines
Authors: Raindlová, Veronika ; Hocek, Michal
Document type: Papers
Conference/Event: Chemistry of Nucleic Acid Components /15./, Český Krumlov (CZ), 2011-06-05 / 2011-06-10
Year: 2011
Language: eng
Abstract: A new simple methodology for DNA conjugation or staining was developed. 2′-Deoxyribonucleoside triphosphates (dNTPs) bearing reactive aldehyde group were prepared by one-step Suzuki cross-coupling reaction of halogenated dNTPs with boronic acid. These modified dNTPs were enzymatically incorporated into DNA by primer extension (PEX) or amplified by polymerase chain reaction (PCR) using different DNA polymerases. The followup reaction between aldehyde-modified PCR products and hydrazine derivatives gave coloured DNA conjugated hydrazones. This methodology was also used for further conjugations of aldehyde-modified 2′-deoxyribonucleoside monophosphates (dNMPs) with amines by reductive amination.
Keywords: aldehydes; bioconjugation; DNA
Project no.: CEZ:AV0Z40550506 (CEP), LC512 (CEP), GA203/09/0317 (CEP)
Funding provider: GA MŠk, GA ČR
Host item entry: Chemistry of Nucleic Acid Components. 15th Symposium, ISBN 978-80-86241-37-1

Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0198862

Permalink: http://www.nusl.cz/ntk/nusl-55839


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Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers
 Record created 2011-09-06, last modified 2024-01-26


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