Název:
Analysis of lipids mobilized by adipokinetic hormones in the firebug Pyrrhocoris apterur (Heteroptera: Pyrrhocoridae)
Autoři:
BÁRTŮ, Iva Typ dokumentu: Rigorózní práce
Rok:
2010
Jazyk:
eng
Abstrakt: An effect of two adipokinetic hormones (Pyrap-AKH and Peram-CAH-II) on distribution of diacylglycerol (DG) molecular species and their fatty acid (FA) constituents were investigated by liquid chromatography (HPLC) - electrospray ionization mass spectrometry (ESI-MS) in haemolymph of the firebug Pyrrhocoris apterus. The results show that DGs with characteristic FAs are preferentially mobilized from fat body (FB) by the action of both AKHs produced by P. apterus. Both the macropterous and brachypterous morphs show very close DG and FA profiles. A separate action of the Pyrap-AKH and the Peram-CAH-II, however, exerts distinct differences in FA distribution at the macropterous morph. It seems that partially C16 and mainly unsaturated C18 FAs play a dominant role in the AKH action with an exclusive position of linoleic acid (18:2) which represents 50 - 60% of the total DG mobilization. The metabolically active C16 and C18 FAs are preferentially absorbed from the linden seeds and accumulated in the FB.
Klíčová slova:
AKH; diacylglycerol; ESI-MS; fat body; fatty acid; haemolymph; Heteroptera; HPLC; Insect; linden seed; lipid; mass spectrometry; Pyrrhociris apterus; triacylglycerol Citace: BÁRTŮ, Iva. Analysis of lipids mobilized by adipokinetic hormones in the firebug Pyrrhocoris apterur (Heteroptera: Pyrrhocoridae). České Budějovice, 2010. rigorózní práce (RNDr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Instituce: Jihočeská univerzita v Českých Budějovicích
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