Název: Effects of various number of basal and covering polyelectrolyte layers on immobilized enzyme activity
Překlad názvu: Effects of various number of basal and covering polyelectrolyte layers on immobilized enzyme activity
Autoři: SHOJAEI, Seyedeh Sara
Typ dokumentu: Bakalářské práce
Rok: 2022
Jazyk: cze
Abstrakt: This thesis is focused on the immobilization of a selected biomolecule, Horseradish peroxidase enzyme (HRP), into a polyelectrolyte multilayer on the glass surface in order to investigate HRP stability and activity in time and specific conditions. To accomplish this work, layer-by-layer deposition approach is employed to fabricate a multilayer of polystyrene sulfonate (PSS) and polyallylamine hydrochloride (PAH). Therefore, 0.1 ěg of HRP enzyme is immobilized onto the various constructs with different number of basal and covering PSS and PAH polyelectrolyte layers. As a results, the activity of the enzyme is increased by further underlying PSS and PAH polyelectrolyte layers. The enzyme activity of the constructs which are not covered by PSS/PAH polyelectrolyte layers is diminished, whereas the enzyme activity of constructs which are covered by the PSS/PAH polyelectrolyte layers is increased.
Klíčová slova: Enzyme acitivity; Enzyme immobilazation; Horseradish peroxidase; Layer-by-Layer deposition; Polyelectrolyte layers; Surface modification; Enzyme acitivity; Enzyme immobilazation; Horseradish peroxidase; Layer-by-Layer deposition; Polyelectrolyte layers; Surface modification
Citace: SHOJAEI, Seyedeh Sara. Effects of various number of basal and covering polyelectrolyte layers on immobilized enzyme activity. České Budějovice, 2022. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Instituce: Jihočeská univerzita v Českých Budějovicích (web)
Informace o dostupnosti dokumentu: Plný text není k dispozici.
Původní záznam: http://www.jcu.cz/vskp/59544

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-528153


Záznam je zařazen do těchto sbírek:
Školství > Veřejné vysoké školy > Jihočeská univerzita v Českých Budějovicích
Vysokoškolské kvalifikační práce > Bakalářské práce