Proteiny intermediárních filament bičíkovců ze skupiny Preaxostyla

Švagr, Ezra

5 Abstract Monocercomonoides exilis and Paratrimastix pyriformis are protists from within the group Preaxostyla (Metamonada), they possess an excavate morphology that is presumed to be an ancestral cytoskeletal organisation of eukaryotic cells. A significant part of eukaryotic but specifically excavate morphology is fibers composed of unidentified proteins. The hypothesis on which this thesis builds upon is that these fibers are composed of Intermediate Filament proteins (IF proteins). IF proteins are a polyphyletic group of proteins involved in the assembly of mechano-elastically important fibers in eukaryotes. The most widespread group of these proteins is a family called SF-assemblins, homologues of which were identified first in Chlamydomonas reinhardii, and giardins a protein family first discovered in Giardia intestinalis, which were also found to be related to this group. Nested into the bigger hypothesis is an idea, that SF-assemblins are present in M. exilis and P. pyriformis, further strengthening their position as a universally present eukaryotic feature. The goal of this work was to find support for the morphological hypothesis that LECA possessed an excavate morphology in protein composition of the cytoskeleton. Two approaches were employed. First, identifying proteins in cytoskeletal fraction...

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