Original title: Nanoporous Enzyme-Based Impedimetric Biosensor
Authors: Košelová, Z.
Document type: Papers
Language: eng
Publisher: Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologií
Abstract: The ordered nanopores surfaces has been studied in the field of biosensors for the detection of affinity interactions such as antigen-antibody or nucleic acid hybridization. Nanopores are frequently fabricated by anodic oxidation of aluminium. This method is practically simple method of highly ordered pore formation, but is not suitable for massive production. The principle of detection is usually based on the degree of pore blockage by biomolecular interaction that allows a sensitive electrochemical response. Here we show the development and characterization of the nanopore-based enzymatic biosensor for the detection of analytes. We performed and tested the concept of the simple and versatile method of nanopores formation on the electrode using non-conductive polystyrene nanoparticles. The variability in pore size was modulated by nanoparticles diameter (40 and 80 nm). We measured response of our designed biosensor using electrochemical impedance spectroscopy in presence of redox mediator at low potential. The smaller diameter of nanopores increases charge transfer resistance. Biosensors with 80 nm nanoparticles had behaviour similar to reference without nanoparticles, therefore the pore was too wide for this design. The limit of detection from Rct showed to be at most 0.01 mM for every sample type. This proposed biosensor contributes to the basic knowledge about nanopores sensors, and it could have a potential, for example, in the development of ―point of care‖ devices.
Keywords: electrochemical impedance spectroscopy; enzyme; nanopores; polystyrene nanoparticles
Host item entry: Proceedings I of the 28st Conference STUDENT EEICT 2022: General papers, ISBN 978-80-214-6029-4

Institution: Brno University of Technology (web)
Document availability information: Fulltext is available in the Brno University of Technology Digital Library.
Original record: http://hdl.handle.net/11012/209374

Permalink: http://www.nusl.cz/ntk/nusl-524801

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Universities and colleges > Public universities > Brno University of Technology
Conference materials > Papers
 Record created 2023-05-07, last modified 2023-05-07

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