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	Diffusion Behavior of Analyte Molecules in a Nanoporous Matrix Created from Polystyrene Nanoparticles Košelová, Zuzana This study investigates the diffusion behavior ofanalyte molecules in a nanoporous matrix of polystyrene particles.Two approaches, steady-state current and potential stepchronoamperometry were employed to calculate diffusioncoefficients and compare the effect of nanoporous matrices withdifferent diameter of pores and different method ofimmobilization of enzyme. Pores were created with close packingof polystyrene nanoparticles. The covalent and cross-linkingimmobilization were used to immobilize the enzyme.Chronoamperometric measurement was applied to detect analyte.The study found that the presence of nanoporous matricesimmobilized on p-lysine affects diffusion. The potential stepchronoamperometry method showed the improvement ofdiffusion. This study provides insight into the diffusion behaviorof analyte molecules in nanoporous matrices, which can be usefulin optimizing the design of amperometric biosensors. Detailed record
	Verify the possibility of plasma treatment of printed circuit boards for subsequent surface treatment Brokeš, Josef ; Košelová, Zuzana (referee) ; Otáhal, Alexandr (advisor) This thesis deals with the regeneration and cleaning of printed circuit boards with emphasis on the use of plasma technology. Various surface cleaning methods, including manual cleaning, industrial washing and plasma treatment, were investigated and their effect on the subsequent quality of the paintwork. A systematic analysis of the effect of plasma treatment on the paint quality and functionality of the plates is also carried out, including an investigation of the possibility of regenerating older plates. This study provides a basis for further research and development in this area. The thesis also presents the design and implementation of a prototype plasma developer device for integration into a production line. Detailed record
	Nanoporous Enzyme-Based Impedimetric Biosensor Košelová, Z. The ordered nanopores surfaces has been studied in the field of biosensors for the detection of affinity interactions such as antigen-antibody or nucleic acid hybridization. Nanopores are frequently fabricated by anodic oxidation of aluminium. This method is practically simple method of highly ordered pore formation, but is not suitable for massive production. The principle of detection is usually based on the degree of pore blockage by biomolecular interaction that allows a sensitive electrochemical response. Here we show the development and characterization of the nanopore-based enzymatic biosensor for the detection of analytes. We performed and tested the concept of the simple and versatile method of nanopores formation on the electrode using non-conductive polystyrene nanoparticles. The variability in pore size was modulated by nanoparticles diameter (40 and 80 nm). We measured response of our designed biosensor using electrochemical impedance spectroscopy in presence of redox mediator at low potential. The smaller diameter of nanopores increases charge transfer resistance. Biosensors with 80 nm nanoparticles had behaviour similar to reference without nanoparticles, therefore the pore was too wide for this design. The limit of detection from Rct showed to be at most 0.01 mM for every sample type. This proposed biosensor contributes to the basic knowledge about nanopores sensors, and it could have a potential, for example, in the development of ―point of care‖ devices. Detailed record

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1	Košelová, Zuzana

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