Original title: Isotachophoretic determination of ketone bodies and lactate in cattle serum
Authors: Vyskočilová, V. ; Pechová, A. ; Pantůčková, Pavla
Document type: Papers
Conference/Event: CECE 2014. International Interdisciplinary Meeting on Bioanalysis /11./, Brno (CZ), 2014-10-20 / 2014-10-22
Year: 2014
Language: eng
Abstract: The aim of the work was the optimization of isotachophoretic determination of lactate and ketone bodies (beta-hydroxybutyrate and acetoacetate) for veterinary medicine. The measurement of these metabolites is very important for the evaluation of metabolic diseases of cattle. The complete separation of these three analytes together with other possible blood components was achieved using 10 mmol/l hydrochloric acid with beta-alanine as a leading electrolyte at pH = 3.15 and 10 mmol/l sodium propionate as a terminating electrolyte. Limits of detection determined from the standard mixture for acetoacetate, lactate and beta-hydroxybutyrate were 3.75 mu mol/l, 3.96 mu mol/l and 7.46 mu mol/l, respectively.
Keywords: cattle serum; isotachophoresis; ketone bodies; lactate
Host item entry: CECE 2014. 11th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-2-0

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available at external website.
External URL: http://www.ce-ce.org/user_uploads/history/CECE2014_Brno/CECE_2014_proceedings_full.pdf
Original record: http://hdl.handle.net/11104/0297961

Permalink: http://www.nusl.cz/ntk/nusl-508142


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Research > Institutes ASCR > Institute of Analytical Chemistry
Conference materials > Papers