Original title: Alkaloidy Papaver rhoeas L. (Papaveraceae) a jejich biologická aktivita vztažená k Alzheimerově chorobě III.
Translated title: Alkaloids of Papaver rhoeas L. (Papaveraceae) and their biological activity related to Alzheimerʼs disease III.
Authors: Bučková, Anna ; Chlebek, Jakub (advisor) ; Suchánková, Daniela (referee)
Document type: Master’s theses
Year: 2022
Language: cze
Abstract: [cze] [eng]
Alkaloidy Papaver rhoeas L. (Papaveraceae) a jejich biologická aktivita vztažená k Alzheimerově chorobě III. ABSTRAKT Bučková, A.: Alkaloidy Papaver rhoeas L. (Papaveraceae) a jejich biologická aktivita vztažená k Alzheimerově chorobě III.; Diplomová práce, Univerzita Karlova, Farmaceutická fakulta v Hradci Králové, Katedra farmakognozie a farmaceutické botaniky, Hradec Králové 2022, počet stran 59 Tato diplomová práce se zabývá separací spojeného výtřepku (CHCl3 +EtOAc + I- ) Papaver rhoeas L. flash chromatografií a následnou izolací alkaloidů z vybraných frakcí preparativní TLC. Objasnění struktury izolovaných látek probíhalo na základě spektrometrických a spektroskopických metod (MS, NMR a optické otáčivosti). Alkaloidy byly identifikované jako (+)-rhoeadin, (+)-rhoeagenin a (+)-isorhoeagenin. Po objasnění struktury byly alkaloidy testovány in vitro na schopnost inhibovat enzymy hrající roli v patogenezi Alzheimerovy choroby (acetylcholinesterasa, butyrylcholinesterasa a prolyloligopeptidasa). Na základě získaných výsledků se studované alkaloidy jeví jako neaktivní vůči vybraným enzymům (hodnoty IC50 > 100 µM). Klíčová slova: Papaver rhoeas, Papaveraceae, Alzheimerova choroba, acetylcholinesterasa, butyrylcholinesterasa, prolyloligopeptidasa Alkaloids of Papaver rhoeas L. (Papaveraceae) and their biological activity related to Alzheimerʼs disease III. ABSTRACT Bučková, A.: Alkaloids of Papaver rhoeas L. (Papaveraceae) and their biological activity related to Alzheimerʼs disease III.; Diploma thesis, Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmacognosy and Pharmaceutical Botany, Hradec Králové 2022, 59 pages The aim of this diploma thesis was to fractionate the combined extract (CHCl3 +EtOAc + I- ) from Papaver rhoeas L. using flash chromatography and subsequently, to isolate alkaloids from the chosen factions by preparative TLC. The chemical structures of the isolated compounds were determined by spetrometric and spectroscopic methods (MS, NMR and optical rotation). Alkaloids were identified as (+)-rhoeadin, (+)-rhoeagenin a (+)-isorhoeagenin. After structure elucidation, the alkaloids were tested in vitro for inhibitory activity towards enzymes that are playing part in the pathogenesis of the Alzheimer's disease (acetylcholinesterase, butyrylcholinesterase a prolyloligopeptidase). Based on the results, the studied alkaloids seem to be inactive towards chosen enzymes (values IC50 > 100 µM). Key words: Papaver rhoeas, Papaveraceae, Alzheimerʼs disease, acetylcholinesterase, butyrylcholinesterase, prolyl...
Keywords: acetylcholinesterase; Alzheimerʼs disease; butyrylcholinesterase; isoquinoline alkaloids; Papaver rhoeas; Papaveraceae; prolyl oligopeptidase; acetylcholinesterasa; Alzheimerova choroba; butyrylcholinesterasa; isochinolinové alkaloidy; Papaver rhoeas; Papaveraceae; prolyloligopeptidasa

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/174453

Permalink: http://www.nusl.cz/ntk/nusl-507128


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Academic theses (ETDs) > Master’s theses