Original title: Analýza historických léčivých přípravků s obsahem alkaloidů, sulfonamidu, derivátů barbiturové kyseliny a derivátu pyrazolu
Translated title: Analysis of Historical Pharmaceutical Preparations Containing Alkaloids, Sulphonamide, Derivatives of Barbituric Acid, and Derivative of Pyrazolone
Authors: Kudláček, Karel ; Nesměrák, Karel (advisor) ; Bosáková, Zuzana (referee)
Document type: Master’s theses
Year: 2016
Language: cze
Abstract: [cze] [eng]
Léčivé přípravky obsahující chinin (injekční roztoky), sulfanilamid, aminofenazon, barbital (tablety), kofein, fenobarbital (dražé) a theofylin (čípek) staré sedmdesát let byly analyzovány pomocí RP-HPLC. Kvanti-fikace probíhala metodou HPLC-UV a UV-spektrofotometrií. Degradační produkty byly detegovány metodami HPLC- UV, a HPLC-MS. Separační podmínky byly optimalizovány. Chininové vzorky obsahují 92 %, resp. 87 % deklarovaného obsahu chininu. Chinotoxin byl identifikován jako rozpadový produkt chininu. Koncentrace theofylinu a kofeinu v příslušných formách zůstala po letech nezměněna. Nižší množství účinné látky (pokles o 8-22 %) bylo nalezeno v přípravcích obsahující sulfanilamid, barbital, fenobarbital a aminofenazon. Degradační produkty nebyly u těchto léčiv nalezeny. Pharmaceutical preparations of quinine (injection solutions), sulfanilamide, aminophenazone, barbital (tablets), caffeine, phenobarbital (dragee), and theophylline (suppositories) about seventy years old were analyzed using RP-HPLC. Samples were quantified by HPLC-UV and UV-spectrophotometry. Products of degradation were identified using HPLC-UV and HPLC-MS. Conditions of separation were optimized. The samples of quinine injection solutions consist of 92% or 87% of declared quinine content. Quinotoxine has been identified as the product of quinine degradation. The quantification of theophylline in suppositories and caffeine in dragee did not show any degradation after more than 67 years from their manufacturing. Decrase of potent amount (decrase about 8-22 %) were found in drugs containing sulfanilamide, barbital, phenobarbital and aminophenazone. Products of degradation of these pharmaceuticals were not found.
Keywords: degradation; long-term stability; pharmaceuticals; RP-HPLC; spectrometry; degradace; dlouhodobá stabilita; léčiva; RP-HPLC; spektrometrie

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/75929

Permalink: http://www.nusl.cz/ntk/nusl-490544


The record appears in these collections:
Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses