Original title: Termodynamika tvorby DNA vlásenek
Translated title: The thermodynamics of DNA-hairpin formation
Authors: Sgallová, Ráchel ; Římal, Václav (advisor) ; Profant, Václav (referee)
Document type: Master’s theses
Year: 2019
Language: cze
Abstract: [cze] [eng]
Serum response factor (SRF) je transkripèní faktor vá¾ící se na vysoce konzer- vovanou sekvenci DNA, tzv. CArG box. Vzhledem k sekvenci nukleotidù CArG boxu by mohlo docházet k tvorbì vlásenek, resp. kruciformy. V rámci této di- plomové práce byla pomocí jaderné magnetické rezonance studována struktura CArG boxu v lidském genu c-fos. Byla zmìøena 1 H spektra v teplotním rozsahu 274{356 K a dvourozmìrná spektra 1 H{1 H NOESY a 1 H{13 C HMBC sekvencí DNA o délce 12, 14 a 16 nukleotidù. Z namìøených teplotních závislostí byly zís- kány termodynamické parametry tvorby sekundárních struktur ve zkoumaných vzorcích. Na základì spekter NOESY a nezávislosti teploty tání na koncentraci byla potvrzena tvorba vlásenek ve zkoumaných vzorcích. Odli¹nost sekundární struktury od B{DNA by mohla být vysvìtlením vysoké a nity SRF k CArG boxu. 1 Serum response factor (SRF) is a transcription factor which binds to a highly conserved DNA sequence called the CArG box. According to the nucleotide sequence of CArG box it could form a hairpin structure or a cruciform. In this master thesis, the structure of the CArG box in a human gene c-fos was studied by nuclear magnetic resonance. 1 H spectra at temperatures 274{356 K, two-dimensional 1 H{1 H NOESY spectra, and two-dimensional 1 H{13 C HMBC spectra for DNA sequences with lengths of 12, 14 and 16 nucleoti- des were acquired. The thermodynamic parameters of formation of the secondary structure in the samples were determined from the measured temperature depen- dencies. The hairpin formation in the samples was con rmed based on the NOESY spectra and the lack of dependency of the melting temperature on concentration. The observed difference of the secondary structure from B-DNA could serve as a possible explanation of the high a nity of SRF to CArG box. 1
Keywords: CArG box; DNA; hairpin; NMR; CArG box; DNA; NMR; vlásenka

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/107743

Permalink: http://www.nusl.cz/ntk/nusl-487925


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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses