Název:
My_Umi_Tool: Processing Of Unique Molecular Identifiers Without Mapping To A Reference Genome
Autoři:
Barilíková, Lujza Typ dokumentu: Příspěvky z konference
Jazyk:
eng
Nakladatel: Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologií
Abstrakt:
The presented paper describes a novel algorithm for processing unique molecular identifiers (UMIs) without mapping to a reference genome. For the past few years, there has been a rapid rise in the use of sequencing technologies and progress in bioinformatics tools. However, available tools for processing UMIs are usually relative computationally demanding and time-consuming which led us to design and develop a new algorithm as well as to implement some available software to provide an effective estimation of the total number of unique molecules. My_UMI_tool performs four major steps: preprocessing of an input file, clustering by UMIs, clustering by sequence similarity, and marking duplicates to generate final file.
Klíčová slova:
next-generation sequencing (NGS); PCR error; unique molecular identifiers (UMIs) Zdrojový dokument: Proceedings II of the 26st Conference STUDENT EEICT 2020: Selected papers, ISBN 978-80-214-5868-0
Instituce: Vysoké učení technické v Brně
(web)
Informace o dostupnosti dokumentu:
Plný text je dostupný v Digitální knihovně VUT. Původní záznam: http://hdl.handle.net/11012/200625