Retrovirové varianty a jejich vztah k nepermisivním buňkám

Přikryl, David

In my doctoral thesis, I focused on studying the envelope glycoprotein of ALV (Avian Leukosis Viruses), a group of retroviruses infecting Galliformes and inducing a variety of diseases. Eradication of these viruses in the farming industry is in progress and information about the virus spread, namely corresponding receptors, is needed to successfully accomplish this feat. Furthermore, many variants of retroviruses infect cells regardless of the absence of the corresponding receptor, and understanding of this phenomenon is also crucial. We analyzed a newly emerged subgroup of ALV, termed K for its sequence divergence from other subgroups, and determined its host range, interference, and receptor usage, to confirm whether this group deserves a new letter for its designation. We identified a receptor of ALV-K that proved to be Tva, the receptor also used by ALV-A. However, since the K subgroup differs from the A subgroup by its host range and inhibition by the soluble form of Tva, we expect the two subgroups use different epitopes of the Tva receptor. We also analyzed a variant of ALV-C exhibiting an extended host range as it successfully infected hamster cells. We found that the extended host range correlates with the ability of the envelope glycoprotein to acquire activated prefusion state...

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