Original title: Application of morphology filters to compensation of lateral illumination inhomogeneities in confocal microscopy images
Authors: Michálek, Jan ; Čapek, Martin ; Mao, X. W. ; Kubínová, Lucie
Document type: Papers
Conference/Event: International EURASIP Conference Biosignal /20./, Brno (CZ), 2010-06-27 / 2010-06-29
Year: 2010
Language: eng
Abstract: Due to multiple distortion effects, confocal laser scanning microscopy (CLSM) images of even homogeneous specimen regions suffer from irregular brightness variations. In this paper, a fast correction method based on estimating a spatially variable illumination gain, and multiplying acquired CLSM images by the inverse of the estimated gain, is presented. The method does not require any special calibration (reference) images since the gain estimate is extracted from the CLSM image being corrected itself. The proposed approach exploits two types of morphological filters: the median filter and a morphological operator called the upper Lipschitz cover
Keywords: CLSM; illumination inhomogeneities
Project no.: CEZ:AV0Z50110509 (CEP), GA102/08/0691 (CEP), GA304/09/0733 (CEP), LC06063 (CEP), MEB090910 (CEP)
Funding provider: GA ČR, GA ČR, GA MŠk, GA MŠk
Host item entry: Analysis of biomedical signals and images, ISBN 978-80-214-4106-4, ISSN 1211-412X

Institution: Institute of Physiology AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0187897

Permalink: http://www.nusl.cz/ntk/nusl-41650


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Research > Institutes ASCR > Institute of Physiology
Conference materials > Papers