Original title: Nanofiber scaffolds for local delivery of stem cells and immunosuppressive drugs for therapeutic purposes
Authors: Zajícová, Alena ; Kössl, Jan ; Heřmánková, Barbora ; Boháčová, Pavla ; Holáň, Vladimír
Document type: Papers
Conference/Event: International Conference on Nanomaterials - Research and Application (NANOCON) /9./, Brno (CZ), 20171018
Year: 2018
Language: eng
Abstract: Cell-based therapy of local tissue injuries or damages requires application of stem cells and inhibition of harmful inflammatory reaction which could impede the healing process. To increase the effectiveness of this therapy, a local administration of drugs can avoid their side effects associated with a systemic treatment. A local therapy requires suitable carriers, which can transfer the cells and drugs to the site of injury. As a promising carriers turned out nanofiber scaffolds prepared by electrospinning technology from various types of polymers. The main advantage of this technology is a possibility to define properties of nanofiber scaffolds, optimal for the growth and transfer of stem cells, and which could incorporate various types of immunosuppressive drugs. Here we describe the formation and use of nanofiber scaffolds prepared by needleless electrospinning technology from poly (L-lactic acid) (PLA) which are loaded with immunosuppressive drug Cyclosporine A (CsA). We show that CsA-loaded nanofibers effectively and selectively inhibit proliferation of activated T cells and suppress the production of T cell cytokines in vitro. Simultaneously, these nanofiber scaffolds enable growth of mesenchymal stem cells (MSCs) and thus can serve as stem cell carriers. Moreover, using an experimental mouse model of skin transplantation, we showed that covering skin allografts with MSC-seeded and CsA-loaded nanofibers significantly inhibited the local production of pro-inflammatory cytokines IL-2, IL-17 and IFN-gamma, and supported healing. Thus, nanofiber scaffolds seeded with stem cells and loaded with CsA can serve as carriers of cells and drugs for a local cell therapy and for simultaneous effective immunosuppression.
Keywords: cytokines; immunosuppressive drug CsA; nanofiber scaffold; stem cells
Project no.: LO1508 (CEP)
Funding provider: GA MŠk
Host item entry: Nanocon 2017 : conference proceedings : 9th International Conference on Nanomaterials - Research & Application, ISBN 9788087294819

Institution: Institute of Experimental Medicine AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0302803

Permalink: http://www.nusl.cz/ntk/nusl-408928


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Research > Institutes ASCR > Institute of Experimental Medicine
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