Original title: Labeling of peptides by Na125I-IODO-GENtm system
Authors: Veselá, Iva ; Elbert, Tomáš
Document type: Papers
Conference/Event: Biologically Active Peptides. Conference /11./, Praha (CZ), 2009-04-22 / 2009-04-24
Year: 2009
Language: eng
Abstract: Very high excess of IODO-GEN(tm) reagent (115 nmol in commercially available precoated tubes from Pierce) leads to damage of oxidation sensitive peptides containing tryptophan residues. Using 5 to 23 nmol of IODO-GEN(tm) in a reaction with 1 to 4 mCi of Na[125-I] and 10 to 30 nmol of peptide leads to reproducible results and reproducible yields.
Keywords: 125-I; IODO-GEN; peptides; radioiodination
Project no.: CEZ:AV0Z40550506 (CEP)
Host item entry: Biologically Active Peptides. 11th Conference, ISBN 978-80-86241-31-9

Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0177765

Permalink: http://www.nusl.cz/ntk/nusl-40737


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Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers