Original title:
Labeling of peptides by Na125I-IODO-GENtm system
Authors:
Veselá, Iva ; Elbert, Tomáš Document type: Papers Conference/Event: Biologically Active Peptides. Conference /11./, Praha (CZ), 2009-04-22 / 2009-04-24
Year:
2009
Language:
eng Abstract:
Very high excess of IODO-GEN(tm) reagent (115 nmol in commercially available precoated tubes from Pierce) leads to damage of oxidation sensitive peptides containing tryptophan residues. Using 5 to 23 nmol of IODO-GEN(tm) in a reaction with 1 to 4 mCi of Na[125-I] and 10 to 30 nmol of peptide leads to reproducible results and reproducible yields.
Keywords:
125-I; IODO-GEN; peptides; radioiodination Project no.: CEZ:AV0Z40550506 (CEP) Host item entry: Biologically Active Peptides. 11th Conference, ISBN 978-80-86241-31-9
Institution: Institute of Organic Chemistry and Biochemistry AS ČR
(web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences. Original record: http://hdl.handle.net/11104/0177765