Original title: Electrochemical Detection of p53 Protein Interactions with Plasmid DNAs Modified with Cisplatin Using Immunoprecipitation at Magnetic Microbeads
Authors: Pivoňková, Hana ; Tichý, Vlastimil ; Orság, Petr ; Šebest, Peter ; Fojta, Miroslav
Document type: Papers
Conference/Event: Moderní Elektrochemické Metody /34./, Jetřichovice (CZ), 2014-05-19 / 2014-05-23
Year: 2014
Language: eng
Abstract: Antineoplastic drug [cis-diamminedichloroplatinum(II)] (cisplatin) forms covalent adducts with DNA. Cisplatin-modified DNA can be determined sensitively using square-wave voltammetry at mercury electrodes. Tumor suppressor protein p53 binds to DNA in different modes, including sequence-and structure-specific ones and these interactions are influenced by modification of the DNA with cisplatin. In this contribution we present a simple immunoprecipitation technique with magnetic beads, followed by voltammetric determination of recovered cisplatinated DNA, for the evaluation of p53 protein binding to DNAs containing various target sites differing in their proneness to being internally modified with the platinum complex.
Keywords: binding; platinum; recognition
Host item entry: XXXIV. Moderní elektrochemické metody sborník přednášek, ISBN 978-80-905221-2-1

Institution: Institute of Biophysics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0297924

Permalink: http://www.nusl.cz/ntk/nusl-396438


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Research > Institutes ASCR > Institute of Biophysics
Conference materials > Papers