Original title: DNA POLYMERASE STOP ASSAY FOR DETECTION OF G-QUADRUPLEXES
Authors: Petr, Marek ; Bažantová, Pavla ; Adámik, Matěj ; Kejnovská, Iva ; Dvořáková, Zuzana ; Vorlíčková, Michaela ; Pečinka, Petr ; Brázdová, Marie
Document type: Papers
Conference/Event: CECE 2014. International Interdisciplinary Meeting on Bioanalysis /11./, Brno (CZ), 2014-10-20 / 2014-10-22
Year: 2014
Language: eng
Abstract: In this work we performed DNA polymerase stop assays while using template DNA derived from promoter regions of VEGF and c-Myc proto-oncogenes under variety of experimental conditions. Partial stopping of DNA synthesis along the template strand was observed in the presence of K+ ions due to formation of stable G-quadruplex structures. In contrast, Na+ ions alone were unable to stabilize G-quadruplexes to stop the reaction at their site. These data suggest that K+ and Na+ ions, which are both known to stabilize G-quadruplexes, do this in different manner or extent.
Keywords: DNA
Host item entry: CECE 2014. 11th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-2-0

Institution: Institute of Biophysics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0297919

Permalink: http://www.nusl.cz/ntk/nusl-396436


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Research > Institutes ASCR > Institute of Biophysics
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